유세포 측정기를 이용한 DNA 히스토그램의 해석;대장 및 직장암종 신선조직과 파라핀포매조직간에서의 DNA 히스토그램 비교

Abstract

As flow cytometric analysis using paraffin-embedded tissue we developed by Hedley et al in 1983, retrospective study with large amount of archival material was possible. Many literatures reported that the result of paraffin embedded tissue was compatible with that of fresh tissue. We compared the DNA histograms of 26 cases of colorectal adenocarcinoma in which the analysis was done in both flesh and paraffin-embedded tissues Aneuploidy in flesh and paraffin-embedded tissues was 73.0% and 50.0%, respectively. The concordance rate of fresh and paraffin-embedded tissues was 76.8% and six interpreters were agreed in 73.0% of the cases. Because flow cytometry DNA analysis using fresh tissue can detect more aneuploid population than in paraffin-embedded tissue. the formed is strongly recommened in DNA ploidy study. Also careful observation using standard criteria may improve the interpretation of DNA histogram.