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Nuclear protein binding patterns in the 5'-upstream regulatory elements of HLA class I genes
DC Field | Value | Language |
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dc.contributor.author | 김세종 | - |
dc.contributor.author | 박전한 | - |
dc.date.accessioned | 2023-07-12T00:11:00Z | - |
dc.date.available | 2023-07-12T00:11:00Z | - |
dc.date.issued | 1994-09 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/194920 | - |
dc.description.abstract | The expression of MHC class I genes has been thought to be regulated by two major cis-acting regulatory elements. The first region, enhancer A (Enh A) spanning from positions -210 to -165 contains perfect palindrome (PP), TGGGGATTCCCCA. The PP is well-conserved both in mouse and human MHC class I genes, even though the PP is disrupted by 2 bp substitutions (TGAGGATTCTCCA) in HLA-C genes. Three proteins binding to the Enh A of HLA-A and -B locus genes, but very weakly or nearly not to the Enh A of HLA-C locus gene have been identified. To determine functional importance of the PP for binding of trans-acting protein, mutant DNA probes were made by site-directed in vitro mutagenesis and then electrophoretic mobility shift assay was performed. HLA-A mutant DNA probe, in which the PP is disrupted, shows the same nuclear protein binding pattern as that of the HLA-C gene, and HLA-C mutant DNA probe, in which the PP is introduced, shows the same nuclear protein binding pattern as that of the wild type HLA-A gene. These data suggest that the perfect palindrome and its cognate DNA binding nuclear protein play an important role in the HLA class I gene regulation, and thus the lower expression of HLA-C antigen may be ascribed to no or very weak factor binding to the nonpalindromic sequences of HLA-C upstream DNA. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Cell Nucleus / metabolism | - |
dc.subject.MESH | Enhancer Elements, Genetic | - |
dc.subject.MESH | Genes, MHC Class I* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nuclear Proteins / metabolism* | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Regulatory Sequences, Nucleic Acid* | - |
dc.title | Nuclear protein binding patterns in the 5'-upstream regulatory elements of HLA class I genes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Jeon Han Park | - |
dc.contributor.googleauthor | Joo Deuk Kim | - |
dc.contributor.googleauthor | Se Jong Kim | - |
dc.identifier.doi | 10.3349/ymj.1994.35.3.295 | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A01641 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 7975738 | - |
dc.contributor.alternativeName | Kim, Se Jong | - |
dc.contributor.affiliatedAuthor | 김세종 | - |
dc.contributor.affiliatedAuthor | 박전한 | - |
dc.citation.volume | 35 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 295 | - |
dc.citation.endPage | 307 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.35(3) : 295-307, 1994-09 | - |
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