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Cell-based analysis of pairwise interactions between the components of the multi-tRNA synthetase complex
DC Field | Value | Language |
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dc.date.accessioned | 2023-07-03T02:09:46Z | - |
dc.date.available | 2023-07-03T02:09:46Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/194846 | - |
dc.description.abstract | Cytoplasmic aminoacyl-tRNA synthetases (ARSs) are organized into multi-tRNA synthetase complexes (MSCs), from Archaea to mammals. An evolutionary conserved role of the MSCs is enhancement of aminoacylation by forming stable associations of the ARSs and tRNAs. In mammals, a single macromolecular MSC exists, which is composed of eight cytoplasmic ARSs, for nine amino acids, and three scaffold proteins. Consequently, nearly half of aminoacyl-tRNA efflux becomes concentrated at the MSC. Stable supply of aminoacyl-tRNA to the ribosome is, therefore, considered to be a major role of the mammalian MSC. Furthermore, the mammalian MSC also serves as a reservoir for releasable components with noncanonical functions. In this study, a split-luciferase complementation system was applied to investigate the configuration of the MSC in live mammalian cells. Multiplex interconnections between the components were simplified into binary protein-protein interactions, and pairwise comparison of the interactions reconstituted a framework consistent with previous in vitro studies. Reversibility of the split-luciferase reporter binding demonstrated convertible organization of the mammalian MSC, including interferon gamma (IFNγ)-stimulated glutamyl-prolyl-tRNA synthetase 1 (EPRS1) release, as well as the cooperation with the ribosome bridged by the tRNAs. The cell-based analysis provided an improved understanding of the flexible framework of the mammalian MSC in physiological conditions. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | The Federation | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Amino Acyl-tRNA Synthetases / metabolism* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CHO Cells | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cricetulus | - |
dc.subject.MESH | Interferon-gamma / metabolism | - |
dc.subject.MESH | Mammals / metabolism | - |
dc.subject.MESH | Protein Interaction Maps / physiology | - |
dc.subject.MESH | Ribosomes / metabolism | - |
dc.title | Cell-based analysis of pairwise interactions between the components of the multi-tRNA synthetase complex | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Others | - |
dc.contributor.googleauthor | Jiwon Kong | - |
dc.contributor.googleauthor | Sunghoon Kim | - |
dc.identifier.doi | 10.1096/fj.202000418R | - |
dc.relation.journalcode | J00889 | - |
dc.identifier.eissn | 1530-6860 | - |
dc.identifier.pmid | 32539228 | - |
dc.identifier.url | https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000418R | - |
dc.subject.keyword | aminoacyl-tRNA synthetase | - |
dc.subject.keyword | macromolecular complex | - |
dc.subject.keyword | mammalian multi-tRNA synthetase complex | - |
dc.subject.keyword | ribosome | - |
dc.subject.keyword | tRNA | - |
dc.citation.volume | 34 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 10476 | - |
dc.citation.endPage | 10488 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, Vol.34(8) : 10476-10488, 2020-08 | - |
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