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Clinical Usefulness of the QMAC-dRAST System for AmpC β-lactamase-Producing Enterobacterales

Authors
 Heekang Choi  ;  Daewon Kim  ;  Mijung Kwon  ;  Jung-Hyun Byun  ;  Bonghwan Jin  ;  Ki-Ho Hong  ;  Hyukmin Lee  ;  Dongeun Yong 
Citation
 Annals of Clinical Microbiology, Vol.25(4) : 115-125, 2022-12 
Journal Title
Annals of Clinical Microbiology
ISSN
 2288-0585 
Issue Date
2022-12
Keywords
Antimicrobial susceptibility testing ; AmpC β-lactamase ; Bacteremia
Abstract
Background: Rapid antimicrobial susceptibility testing (RAST) is important for the appropriate treatment of bloodstream infections. The QMAC-dRAST system (QuantaMatrix Inc., Korea) can directly perform RAST using positive blood culture samples with microscopic imaging. This study aimed to evaluate the performance of the QMAC-dRAST system for AmpC-β-lactamase-producing Enterobacterales.

Methods: Eighty isolates (20 Morganella morganii, 20 Serratia marcescens, 10 Klebsiella aerogenes, 10 Enterobacter cloacae, and 20 Citrobacter freundii) and 14 antimicrobial agents were included in the antimicrobial susceptibility testing (AST). The performance of the QMAC-dRAST system was evaluated by simulating the clinical blood culturing process. We conducted a comparative evaluation of the QMAC-dRAST and Vitek 2 systems (bioM.rieux Inc., France). Broth microdilution tests were performed as the reference method to resolve any discrepancies in the AST results between the two systems.

Results: For 20 M. morganii and 20 S. marcescens, the categorical agreement (CA) between the QMAC-dRAST and Vitek 2 systems increased from 55.4% to 83.8% after AST algorithm optimization. Moreover, the discrepancy rates decreased as follows: from 19.1% to 5.4% very major errors (VME), from 38.3% to 4.3% major errors (ME), and from 14.6% to 12.1% minor errors (mE) for the QMAC-dRAST system compared to the Vitek 2 system. For all 80 tested isolates, the QMAC-dRAST system showed 93.0% CA, 1.7% VME, 2.3% ME, and 4.9% mE.

Conclusion: The QMAC-dRAST system was comparable to the Vitek 2 system after AST algorithm optimization for AmpC β-lactamase-producers, which are major pathogens and require time to express the enzyme. However, further modifications of the AST algorithm are still warranted.
Files in This Item:
T9992022790.pdf Download
DOI
10.5145/ACM.2022.25.4.1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Daewon(김대원) ORCID logo https://orcid.org/0000-0002-3487-5943
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Hyuk Min(이혁민) ORCID logo https://orcid.org/0000-0002-8523-4126
Hong, Ki Ho(홍기호) ORCID logo https://orcid.org/0000-0002-5700-9036
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/193911
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