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Smartphone-Based SARS-CoV-2 and Variants Detection System using Colorimetric DNAzyme Reaction Triggered by Loop-Mediated Isothermal Amplification (LAMP) with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)

Authors
 Jayeon Song  ;  Baekdong Cha  ;  Jeong Moon  ;  Hyowon Jang  ;  Sunjoo Kim  ;  Jieun Jang  ;  Dongeun Yong  ;  Hyung-Jun Kwon  ;  In-Chul Lee  ;  Eun-Kyung Lim  ;  Juyeon Jung  ;  Hyun Gyu Park  ;  Taejoon Kang 
Citation
 ACS NANO, Vol.16(7) : 11300-11314, 2022-07 
Journal Title
ACS NANO
ISSN
 1936-0851 
Issue Date
2022-07
MeSH
COVID-19* / diagnosis ; Colorimetry ; DNA, Catalytic* / genetics ; Humans ; Molecular Diagnostic Techniques / methods ; Nucleic Acid Amplification Techniques / methods ; SARS-CoV-2 / genetics ; Sensitivity and Specificity ; Smartphone
Keywords
CRISPR-Cas9 ; SARS-CoV-2 ; machine learning ; smartphone ; variants
Abstract
Coronavirus disease (COVID-19) has affected people for over two years. Moreover, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has raised concerns regarding its accurate diagnosis. Here, we report a colorimetric DNAzyme reaction triggered by loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR), referred to as DAMPR assay for detecting SARS-CoV-2 and variants genes with attomolar sensitivity within an hour. The CRISPR-associated protein 9 (Cas9) system eliminated false-positive signals of LAMP products, improving the accuracy of DAMPR assay. Further, we fabricated a portable DAMPR assay system using a three-dimensional printing technique and developed a machine learning (ML)-based smartphone application to routinely check diagnostic results of SARS-CoV-2 and variants. Among blind tests of 136 clinical samples, the proposed system successfully diagnosed COVID-19 patients with a clinical sensitivity and specificity of 100% each. More importantly, the D614G (variant-common), T478K (delta-specific), and A67V (omicron-specific) mutations of the SARS-CoV-2 S gene were detected selectively, enabling the diagnosis of 70 SARS-CoV-2 delta or omicron variant patients. The DAMPR assay system is expected to be employed for on-site, rapid, accurate detection of SARS-CoV-2 and its variants gene and employed in the diagnosis of various infectious diseases.
Files in This Item:
T9992022732.pdf Download
DOI
10.1021/acsnano.2c04840
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/193660
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