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Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Authors
 Hyunjung Cha  ;  Hyeran Kim  ;  Younju Joung  ;  Hyunju Kang  ;  Jeong Moon  ;  Hyowon Jang  ;  Sohyun Park  ;  Hyung-Jun Kwon  ;  In-Chul Lee  ;  Sunjoo Kim  ;  Dongeun Yong  ;  Sun-Woo Yoon  ;  Sung-Gyu Park  ;  Kyeonghye Guk  ;  Eun-Kyung Lim  ;  Hyun Gyu Park  ;  Jaebum Choo  ;  Juyeon Jung  ;  Taejoon Kang 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.202 : 114008, 2022-04 
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
 0956-5663 
Issue Date
2022-04
MeSH
Biosensing Techniques* / methods ; COVID-19* ; Gold ; Humans ; Immunoassay / methods ; Metal Nanoparticles* ; SARS-CoV-2 ; Spectrum Analysis, Raman
Keywords
Antibody ; Coronavirus disease 2019 ; Immunoassay ; Nanoparticle ; Severe acute respiratory syndrome coronavirus 2 ; Surface-enhanced Raman scattering
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.
Files in This Item:
T9992022693.pdf Download
DOI
10.1016/j.bios.2022.114008
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/193464
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