Cited 3 times in
SIRT1 Protects Against Particulate Matter-Induced Oxidative Stress in Human Corneal and Conjunctival Epithelial Cells
DC Field | Value | Language |
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dc.contributor.author | 이형근 | - |
dc.date.accessioned | 2023-03-21T07:29:26Z | - |
dc.date.available | 2023-03-21T07:29:26Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/193400 | - |
dc.description.abstract | Purpose: Sirtuin1 (SIRT1) as a hot therapeutic target for oxidative stress-associated diseases that has been extensively studied. This study aimed to determine the changes in SIRT1 expression in particulate matter (PM)-induced corneal and conjunctival epithelial cell damage and explore potential drugs to reduce PM-associated ocular surface injury. Methods: Immortalized human corneal epithelial cells (HCECs) and human conjunctival epithelial cells (HCjECs) were exposed to an ambient PM sample. Cytotoxicity was evaluated by water-soluble tetrazolium salt-8 assay. SIRT1 expression was measured by Western blot analysis. Reactive oxygen species (ROS) production, cell apoptosis, mitochondrial function, and cell senescence were assessed by using 2',7'-dichlorofluorescein diacetate assay, annexin V apoptosis assay, tetramethylrhodamine ethyl ester assay, and senescence β-galactosidase staining, respectively. Results: PM-induced cytotoxicity of HCECs and HCjECs occurred in a dose-dependent manner. Increased ROS production, as well as decreased SIRT1 expression, were observed in HCECs and HCjECs after 200 µg/mL PM exposure. In addition, PM induced oxidative stress-mediated cellular damage, including cell apoptosis, mitochondrial damage, and cell senescence. Interestingly, SRT1720, a SIRT1 activator, increased SIRT1 expression and decreased ROS production and attenuated PM-induced cell damage in HCECs and HCjECs. Conclusions: This study determined that SIRT1 was involved in PM-induced oxidative stress in HCECs and HCjECs and found that ROS overproduction may a key factor in PM-induced SIRT1 downregulation. The SIRT1 activator, SRT1720, can effectively upregulate SIRT1 expression and inhibit ROS production, thereby reversing PM-induced cell damage. This study provides a new potential target for clinical treatment of PM-associated ocular surface diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Annexin A5 / metabolism | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Epithelial Cells / metabolism | - |
dc.subject.MESH | Esters / metabolism | - |
dc.subject.MESH | Esters / pharmacology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Oxidative Stress | - |
dc.subject.MESH | Particulate Matter* / toxicity | - |
dc.subject.MESH | Reactive Oxygen Species / metabolism | - |
dc.subject.MESH | Sirtuin 1* / metabolism | - |
dc.subject.MESH | Tetrazolium Salts / metabolism | - |
dc.subject.MESH | Tetrazolium Salts / pharmacology | - |
dc.subject.MESH | Water / metabolism | - |
dc.subject.MESH | beta-Galactosidase / metabolism | - |
dc.title | SIRT1 Protects Against Particulate Matter-Induced Oxidative Stress in Human Corneal and Conjunctival Epithelial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Xiangzhe Li | - |
dc.contributor.googleauthor | Boram Kang | - |
dc.contributor.googleauthor | Youngsub Eom | - |
dc.contributor.googleauthor | Jingxiang Zhong | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.contributor.googleauthor | Hyo Myung Kim | - |
dc.contributor.googleauthor | Jong Suk Song | - |
dc.identifier.doi | 10.1167/iovs.63.10.19 | - |
dc.contributor.localId | A03303 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 36169947 | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | 이형근 | - |
dc.citation.volume | 63 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 19 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.63(10) : 19, 2022-09 | - |
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