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Neural Stem Cells Overexpressing Arginine Decarboxylase Improve Functional Recovery from Spinal Cord Injury in a Mouse Model

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dc.contributor.author이종은-
dc.date.accessioned2023-03-03T03:13:50Z-
dc.date.available2023-03-03T03:13:50Z-
dc.date.issued2022-12-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/193005-
dc.description.abstractCurrent therapeutic strategies for spinal cord injury (SCI) cannot fully facilitate neural regeneration or improve function. Arginine decarboxylase (ADC) synthesizes agmatine, an endogenous primary amine with neuroprotective effects. Transfection of human ADC (hADC) gene exerts protective effects after injury in murine brain-derived neural precursor cells (mNPCs). Following from these findings, we investigated the effects of hADC-mNPC transplantation in SCI model mice. Mice with experimentally damaged spinal cords were divided into three groups, separately transplanted with fluorescently labeled (1) control mNPCs, (2) retroviral vector (pLXSN)-infected mNPCs (pLXSN-mNPCs), and (3) hADC-mNPCs. Behavioral comparisons between groups were conducted weekly up to 6 weeks after SCI, and urine volume was measured up to 2 weeks after SCI. A subset of animals was euthanized each week after cell transplantation for molecular and histological analyses. The transplantation groups experienced significantly improved behavioral function, with the best recovery occurring in hADC-mNPC mice. Transplanting hADC-mNPCs improved neurological outcomes, induced oligodendrocyte differentiation and remyelination, increased neural lineage differentiation, and decreased glial scar formation. Moreover, locomotor and bladder function were both rehabilitated. These beneficial effects are likely related to differential BMP-2/4/7 expression in neuronal cells, providing an empirical basis for gene therapy as a curative SCI treatment option.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHCarboxy-Lyases* / genetics-
dc.subject.MESHCarboxy-Lyases* / metabolism-
dc.subject.MESHCell Differentiation / physiology-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHNeural Stem Cells* / metabolism-
dc.subject.MESHNeurons / metabolism-
dc.subject.MESHRecovery of Function-
dc.subject.MESHSpinal Cord / metabolism-
dc.subject.MESHSpinal Cord Injuries* / genetics-
dc.subject.MESHSpinal Cord Injuries* / pathology-
dc.subject.MESHSpinal Cord Injuries* / therapy-
dc.titleNeural Stem Cells Overexpressing Arginine Decarboxylase Improve Functional Recovery from Spinal Cord Injury in a Mouse Model-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anatomy (해부학교실)-
dc.contributor.googleauthorYu Mi Park-
dc.contributor.googleauthorJae Hwan Kim-
dc.contributor.googleauthorJong Eun Lee-
dc.identifier.doi10.3390/ijms232415784-
dc.contributor.localIdA03146-
dc.relation.journalcodeJ01133-
dc.identifier.eissn1422-0067-
dc.identifier.pmid36555425-
dc.subject.keywordagmatine-
dc.subject.keywordarginine decarboxylase-
dc.subject.keywordaxonal re-myelination-
dc.subject.keywordcell transplantation-
dc.subject.keywordfunctional recovery-
dc.subject.keywordgene therapy-
dc.subject.keywordglial scar-
dc.subject.keywordneural progenitor cells-
dc.subject.keywordneurogenesis-
dc.subject.keywordspinal cord injury-
dc.contributor.alternativeNameLee, Jong Eun-
dc.contributor.affiliatedAuthor이종은-
dc.citation.volume23-
dc.citation.number24-
dc.citation.startPage15784-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.23(24) : 15784, 2022-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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