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Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes

Authors
 Jaewook Lee  ;  Ji-Heon Lee  ;  Jagannath Mondal  ;  Joon Hwang  ;  Han Sang Kim  ;  Vinoth Kumar  ;  Akhil Raj  ;  Seung Rim Hwang  ;  Yong-Kyu Lee 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.23(17) : 9619, 2022-08 
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
 1661-6596 
Issue Date
2022-08
MeSH
Biosensing Techniques* / methods ; Exosomes* ; Gold / chemistry ; Graphite* / chemistry ; Humans ; Male ; Nanoparticles* / chemistry ; Neoplasms*
Keywords
binary nanoparticle-decorated graphene ; cancer diagnosis ; cancer-derived exosome ; magnetofluoro-immunoassay ; magnetoplasmonic graphene ; prostate cancer
Abstract
Multi-functionalized carbon nanomaterials have attracted interest owing to their excellent synergic properties, such as plasmon resonance energy transfer and surface-enhanced Raman scattering. Particularly, nanoparticle (NP)-decorated graphene (GRP) has been applied in various fields. In this study, silver NP (AgNP)- and magnetic iron oxide NP (IONP)-decorated GRP were prepared and utilized as biosensing platforms. In this case, AgNPs and GRP exhibit plasmonic properties, whereas IONPs exhibit magnetic properties; therefore, this hybrid nanomaterial could function as a magnetoplasmonic substrate for the magnetofluoro-immunosensing (MFI) system. Conversely, exosomes were recently considered high-potential biomarkers for the diagnosis of diseases. However, exosome diagnostic use requires complex isolation and purification methods. Nevertheless, we successfully detected a prostate-cancer-cell-derived exosome (PC-exosome) from non-purified exosomes in a culture media sample using Ag/IO-GRP and dye-tetraspanin antibodies (Ab). First, the anti-prostate-specific antigen was immobilized on the Ag/IO-GRP and it could isolate the PC-exosome from the sample via an external magnetic force. Dye-tetraspanin Ab was added to the sample to induce the sandwich structure. Based on the number of exosomes, the fluorescence intensity from the dye varied and the system exhibited highly sensitive and selective performance. Consequently, these hybrid materials exhibited excellent potential for biosensing platforms.
Files in This Item:
T202203856.pdf Download
DOI
10.3390/ijms23179619
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Han Sang(김한상) ORCID logo https://orcid.org/0000-0002-6504-9927
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/191836
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