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Development of transgenic models susceptible and resistant to SARS-CoV-2 infection in FVB background mice

DC Field Value Language
dc.contributor.author남기택-
dc.contributor.author신전수-
dc.contributor.author서준영-
dc.date.accessioned2022-12-22T02:50:33Z-
dc.date.available2022-12-22T02:50:33Z-
dc.date.issued2022-07-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/191709-
dc.description.abstractCoronavirus disease (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is currently spreading globally. To overcome the COVID-19 pandemic, preclinical evaluations of vaccines and therapeutics using K18-hACE2 and CAG-hACE2 transgenic mice are ongoing. However, a comparative study on SARS-CoV-2 infection between K18-hACE2 and CAG-hACE2 mice has not been published. In this study, we compared the susceptibility and resistance to SARS-CoV-2 infection between two strains of transgenic mice, which were generated in FVB background mice. K18-hACE2 mice exhibited severe weight loss with definitive lethality, but CAG-hACE2 mice survived; and differences were observed in the lung, spleen, cerebrum, cerebellum, and small intestine. A higher viral titer was detected in the lungs, cerebrums, and cerebellums of K18-hACE2 mice than in the lungs of CAG-hACE2 mice. Severe pneumonia was observed in histopathological findings in K18-hACE2, and mild pneumonia was observed in CAG-hACE2. Atrophy of the splenic white pulp and reduction of spleen weight was observed, and hyperplasia of goblet cells with villi atrophy of the small intestine was observed in K18-hACE2 mice compared to CAG-hACE2 mice. These results indicate that K18-hACE2 mice are relatively susceptible to SARS-CoV-2 and that CAG-hACE2 mice are resistant to SARS-CoV-2. Based on these lineage-specific sensitivities, we suggest that K18-hACE2 mouse is suitable for highly susceptible model of SARS-CoV-2, and CAG-hACE2 mouse is suitable for mild susceptible model of SARS-CoV-2 infection.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherPublic Library of Science-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAngiotensin-Converting Enzyme 2 / genetics-
dc.subject.MESHAnimals-
dc.subject.MESHAtrophy / pathology-
dc.subject.MESHCOVID-19*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHDisease Susceptibility / pathology-
dc.subject.MESHHumans-
dc.subject.MESHLung / pathology-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred Strains-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHPandemics-
dc.subject.MESHPeptidyl-Dipeptidase A-
dc.subject.MESHPneumonia* / pathology-
dc.subject.MESHSARS-CoV-2-
dc.titleDevelopment of transgenic models susceptible and resistant to SARS-CoV-2 infection in FVB background mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorSun-Min Seo-
dc.contributor.googleauthorJae Hyung Son-
dc.contributor.googleauthorJi-Hun Lee-
dc.contributor.googleauthorNa-Won Kim-
dc.contributor.googleauthorEun-Seon Yoo-
dc.contributor.googleauthorAh-Reum Kang-
dc.contributor.googleauthorJi Yun Jang-
dc.contributor.googleauthorDa In On-
dc.contributor.googleauthorHyun Ah Noh-
dc.contributor.googleauthorJun-Won Yun-
dc.contributor.googleauthorJun Won Park-
dc.contributor.googleauthorKang-Seuk Choi-
dc.contributor.googleauthorHo-Young Lee-
dc.contributor.googleauthorJeon-Soo Shin-
dc.contributor.googleauthorJun-Young Seo-
dc.contributor.googleauthorKi Taek Nam-
dc.contributor.googleauthorHo Lee-
dc.contributor.googleauthorJe Kyung Seong-
dc.contributor.googleauthorYang-Kyu Choi-
dc.identifier.doi10.1371/journal.pone.0272019-
dc.contributor.localIdA01243-
dc.contributor.localIdA02144-
dc.contributor.localIdA01911-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid35881617-
dc.contributor.alternativeNameNam, Ki Taek-
dc.contributor.affiliatedAuthor남기택-
dc.contributor.affiliatedAuthor신전수-
dc.contributor.affiliatedAuthor서준영-
dc.citation.volume17-
dc.citation.number7-
dc.citation.startPagee0272019-
dc.identifier.bibliographicCitationPLOS ONE, Vol.17(7) : e0272019, 2022-07-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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