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p53 Immunohistochemistry and Mutation Types Mismatching in High-Grade Serous Ovarian Cancer

 Eunhyang Park  ;  Hyunho Han  ;  Sung-Eun Choi  ;  Hyunjin Park  ;  Ha-Young Woo  ;  Mi Jang  ;  Hyo-Sup Shim  ;  Sohyun Hwang  ;  Haeyoun Kang  ;  Nam-Hoon Cho 
 DIAGNOSTICS, Vol.12(3) : 579, 2022-02 
Journal Title
Issue Date
immunohistochemistry ; next generation sequencing ; oligomerization domain ; p53
High-grade serous carcinoma (HGSCa) of the ovary is featured by TP53 gene mutation. Missense or nonsense mutation types accompany most cases of HGSCa that correlate well with immunohistochemical (IHC) staining results-an all (missense) or none (nonsense) pattern. However, some IHCs produce subclonal or mosaic patterns from which TP53 mutation types, including the wild type of the gene, cannot be clearly deduced. We analyzed a total of 236 cases of ovarian HGSCa and tumors of other histology by matching the results of p53 IHC staining and targeted next-generation sequencing (TruSight Tumor 170 panel). Ambiguous IHCs that do not belong to the conventional "all or none" groups were reviewed to distinguish the true wild type (WT) from potentially pathogenic subclonal or mosaic patterns. There were about 9% of sequencing-IHC mismatching cases, which were enriched by the p53 c-terminal encoding nuclear localization signal and oligomerization domain, in which the subcellular locations of p53 protein were affected. Indeed, mutations in the oligomerization domain of the p53 protein frequently revealed an unmatched signal or cytosolic staining (L289Ffs*57 (Ins), and R342*). We conclude that both mutation types and IHC patterns of p53 are important sources of information to provide a precise diagnosis of HGSCa.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
Yonsei Authors
Park, Eunhyang(박은향) ORCID logo https://orcid.org/0000-0003-2658-5054
Park, Hyunjin(박현진)
Shim, Hyo Sup(심효섭) ORCID logo https://orcid.org/0000-0002-5718-3624
Woo, Ha Young(우하영) ORCID logo https://orcid.org/0000-0002-3078-6484
Jang, Mi(장미) ORCID logo https://orcid.org/0000-0002-0153-9847
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Han, Hyun Ho(한현호) ORCID logo https://orcid.org/0000-0002-6268-0860
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