Cited 16 times in
Preconditioning process for dermal tissue decellularization using electroporation with sonication
DC Field | Value | Language |
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dc.contributor.author | 구민아 | - |
dc.contributor.author | 박종철 | - |
dc.contributor.author | 이미희 | - |
dc.date.accessioned | 2022-12-22T01:17:54Z | - |
dc.date.available | 2022-12-22T01:17:54Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 2056-3418 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191150 | - |
dc.description.abstract | Decellularization to produce bioscaffolds composed of the extracellular matrix (ECM) uses enzymatic, chemical and physical methods to remove antigens and cellular components from tissues. Effective decellularization methods depend on the characteristics of tissues, and in particular, tissues with dense, complex structure and abundant lipid content are difficult to completely decellularize. Our study enables future research on the development of methods and treatments for fabricating bioscaffolds via decellularization of complex and rigid skin tissues, which are not commonly considered for decellularization to date as their structural and functional characteristics could not be preserved after severe decellularization. In this study, decellularization of human dermal tissue was done by a combination of both chemical (0.05% trypsin-EDTA, 2% SDS and 1% Triton X-100) and physical methods (electroporation and sonication). After decellularization, the content of DNA remaining in the tissue was quantitatively confirmed, and the structural change of the tissue and the retention and distribution of ECM components were evaluated through histological and histochemical analysis, respectively. Conditions of the chemical pretreatment that increase the efficiency of physical stimulation as well as decellularization, and conditions for electroporation and sonication without the use of detergents, unlike the methods performed in previous studies, were established to enable the complete decellularization of the skin tissue. The combinatorial decellularization treatment formed micropores in the lipid bilayers of the skin tissues while removing all cell and cellular residues without affecting the ECM properties. Therefore, this procedure can be widely used to fabricate bioscaffolds by decellularizing biological tissues with dense and complex structures. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Oxford University Press in association with the Chinese Society for Biomaterials | - |
dc.relation.isPartOf | REGENERATIVE BIOMATERIALS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Preconditioning process for dermal tissue decellularization using electroporation with sonication | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Min-Ah Koo | - |
dc.contributor.googleauthor | HaKyeong Jeong | - |
dc.contributor.googleauthor | Seung Hee Hong | - |
dc.contributor.googleauthor | Gyeung Mi Seon | - |
dc.contributor.googleauthor | Mi Hee Lee | - |
dc.contributor.googleauthor | Jong-Chul Park | - |
dc.identifier.doi | 10.1093/rb/rbab071 | - |
dc.contributor.localId | A00190 | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A02777 | - |
dc.relation.journalcode | J03101 | - |
dc.identifier.eissn | 2056-3426 | - |
dc.identifier.pmid | 35449827 | - |
dc.subject.keyword | decellularization | - |
dc.subject.keyword | electroporation | - |
dc.subject.keyword | extracellular matrix | - |
dc.subject.keyword | sonication | - |
dc.contributor.alternativeName | Koo, Min-Ah | - |
dc.contributor.affiliatedAuthor | 구민아 | - |
dc.contributor.affiliatedAuthor | 박종철 | - |
dc.contributor.affiliatedAuthor | 이미희 | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | rbab071 | - |
dc.identifier.bibliographicCitation | REGENERATIVE BIOMATERIALS, Vol.9(1) : rbab071, 2022-04 | - |
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