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Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture

Authors
 Chun-Seok Yang  ;  In-Hwan Kim  ;  Hyun-Dong Chae  ;  Dae-Dong Kim  ;  Chang-Ho Jeon 
Citation
 IN VIVO, Vol.35(3) : 1515-1520, 2021-05,06 
Journal Title
IN VIVO
ISSN
 0258-851X 
Issue Date
2021-05
MeSH
Biomarkers, Tumor / genetics ; Cell Culture Techniques ; Colorectal Neoplasms* ; Humans ; Neoplastic Cells, Circulating* ; RNA, Messenger ; Reverse Transcriptase Polymerase Chain Reaction ; Stomach Neoplasms* / genetics
Keywords
Blood ; MAGE ; culture ; hTERT ; neoplasm ; reverse transcription
Abstract
Background/aim: The aim of this study was to detect circulating tumor cells (CTC) in the peripheral blood of gastrointestinal cancer patients using conditionally reprogrammed cell (CRC) culture.

Materials and methods: We confirmed the sensitivity of the CRC culture method. Five ml of blood were obtained from 81 cancer patients (56 colorectal and 25 gastric). The collected mononuclear cells were cultured for 4 weeks in the CRC condition. Finally, cultured cells were characterized by RT-PCR for the expression of hTERT and MAGE A1-6 mRNA.

Results: The CRC method had a CTC detection limit of 6 cells for gastric cancer cells. After culture of 81 blood specimens, 38 formed visible cells, including 5 colonies. Among the 38 cells, 13 were hTERT positive and 4 were MAGE A1-6 positive. The final CTC detection rate was 16.0%.

Conclusion: The CRC culture may potentially be used to evaluate the metastatic cancer cells in the circulation.
DOI
10.21873/invivo.12404
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dae-Dong(김대동)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190959
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