0 259

Cited 15 times in

A catenin of the plakophilin-subfamily, Pkp3, responds to canonical-Wnt pathway components and signals

Authors
 Ji Yeon Hong  ;  Jessica Zapata  ;  Alexandria Blackburn  ;  Ryan Baumert  ;  Seung Min Bae  ;  Hong Ji  ;  Hee Jin Nam  ;  Rachel K Miller  ;  Pierre D McCrea 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.563 : 31-39, 2021-07 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2021-07
MeSH
Animals ; Cells, Cultured ; Humans ; Plakophilins / metabolism* ; Wnt Signaling Pathway ; Xenopus laevis
Keywords
Desmosomal junction ; Desmosome junction ; Destruction complex ; Nucleus ; Pkp-3 ; Plakophilin-3 catenin ; Signaling pool ; Wnt signaling pathway ; plakophilin3-catenin
Abstract
Vertebrate beta-catenin plays a key role as a transducer of canonical-Wnt signals. We earlier reported that, similar to beta-catenin, the cytoplasmic signaling pool of p120-catenin-isoform1 is stabilized in response to canonical-Wnt signals. To obtain a yet broader view of the Wnt-pathway's impact upon catenin proteins, we focused upon plakophilin3 (plakophilin-3; Pkp3) as a representative of the plakophilin-catenin subfamily. Promoting tissue integrity, the plakophilins assist in linking desmosomal cadherins to intermediate filaments at desmosome junctions, and in common with other catenins they perform additional functions including in the nucleus. In this report, we test whether canonical-Wnt pathway components modulate Pkp3 protein levels. We find that in common with beta-catenin and p120-catenin-isoform1, Pkp3 is stabilized in the presence of a Wnt-ligand or a dominant-active form of the LRP6 receptor. Pkp3's levels are conversely lowered upon expressing destruction-complex components such as GSK3β and Axin, and in further likeness to beta-catenin and p120-isoform1, Pkp3 associates with GSK3beta and Axin. Finally, we note that Pkp3-catenin trans-localizes into the nucleus in response to Wnt-ligand and its exogenous expression stimulates an accepted Wnt reporter. These findings fit an expanded model where context-dependent Wnt-signals or pathway components modulate Pkp3-catenin levels. Future studies will be needed to assess potential gene regulatory, cell adhesive, or cytoskeletal effects.
Full Text
https://www.sciencedirect.com/science/article/pii/S0006291X21008147
DOI
10.1016/j.bbrc.2021.05.043
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Hong, Ji Yeon(홍지연)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190867
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links