Cited 11 times in
P62 Links the Autophagy Pathway and the Ubiquitin-Proteasome System in Endothelial Cells during Atherosclerosis
DC Field | Value | Language |
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dc.date.accessioned | 2022-11-24T00:38:18Z | - |
dc.date.available | 2022-11-24T00:38:18Z | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190845 | - |
dc.description.abstract | Among autophagy-related molecules, p62/SQSTM1 is an adaptor for identifying and delivering intracellular cargo for degradation. Since ubiquitination is reversible, it has a switch role in autophagy. Ubiquitination is also involved in regulating autophagy in a timely manner. This study aimed to elucidate how p62-mediated autophagy is regulated in human endothelial cells and macrophages under atherosclerotic conditions, focusing on the lysosomal and proteasomal pathways. Co-cultured HUVECs and THP-1 cells were exposed to oxLDL (50 μg/mL) and autophagy was assessed. To downregulate p62, siRNA was administered, and the E3 ligases were inhibited by Heclin or MLN4924 treatment under the condition that cellular inflammatory processes were stimulated by oxLDL simultaneously initiated autophagy. Downregulating p62 induced an alternative degradation system, and the E3 ligases were found to be involved in the progression of atherosclerosis. Collectively, the present study demonstrated that the endothelial lipid accumulation under atherosclerotic conditions was caused by lysosomal dysfunction associated with autophagy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.publisher | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Atherosclerosis / metabolism | - |
dc.subject.MESH | Atherosclerosis / pathology* | - |
dc.subject.MESH | Autophagy* | - |
dc.subject.MESH | Endothelial Cells / metabolism | - |
dc.subject.MESH | Endothelial Cells / pathology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Proteasome Endopeptidase Complex / genetics | - |
dc.subject.MESH | Proteasome Endopeptidase Complex / metabolism* | - |
dc.subject.MESH | Proteolysis* | - |
dc.subject.MESH | Sequestosome-1 Protein / genetics | - |
dc.subject.MESH | Sequestosome-1 Protein / metabolism* | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Ubiquitin / metabolism | - |
dc.subject.MESH | Ubiquitination* | - |
dc.title | P62 Links the Autophagy Pathway and the Ubiquitin-Proteasome System in Endothelial Cells during Atherosclerosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | SeJeong Kim | - |
dc.contributor.googleauthor | WoongJin Lee | - |
dc.contributor.googleauthor | KyoungJoo Cho | - |
dc.identifier.doi | 10.3390/ijms22157791 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 34360560 | - |
dc.subject.keyword | E3 ligase | - |
dc.subject.keyword | atherosclerosis | - |
dc.subject.keyword | autophagy | - |
dc.subject.keyword | endothelial cell | - |
dc.subject.keyword | oxLDL | - |
dc.citation.volume | 22 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 7791 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.22(15) : 7791, 2021-07 | - |
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