111 366

Cited 3 times in

Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment

Authors
 Seogwon Lee  ;  Ju Yeong Kim  ;  Myung-Hee Yi  ;  In-Yong Lee  ;  Dongeun Yong  ;  Tai-Soon Yong 
Citation
 PLOS ONE, Vol.16(11) : e0257114, 2021-11 
Journal Title
PLOS ONE
Issue Date
2021-11
MeSH
Allergens / isolation & purification* ; Ampicillin / pharmacology* ; Animals ; Anti-Bacterial Agents / pharmacology* ; Aspartic Acid Endopeptidases / isolation & purification* ; Cockroaches / drug effects* ; Cockroaches / microbiology ; Microbiota / drug effects*
Abstract
Purpose: Allergens present in the feces or frass of cockroaches can cause allergic sensitization in humans. The use of fecal and frass extracts for immunotherapy has been previously investigated but has not yet been fully standardized. Here, we treated cockroaches with ampicillin to produce extracts with reduced amounts of total bacteria.

Methods: We performed targeted high-throughput sequencing of 16S rDNA to compare the microbiomes of ampicillin-treated and untreated (control) cockroaches. RNA-seq was performed to identify differentially expressed genes (DEGs) in ampicillin-treated cockroaches.

Results: Analysis of the microbiome revealed that alpha diversity was lower in the ampicillin-treated group than in the control group. Beta diversity analysis indicated that ampicillin treatment altered bacterial composition in the microbiome of cockroaches. Quantitative polymerase chain reaction revealed that almost all bacteria were removed from ampicillin-treated cockroaches. RNA-seq analysis revealed 1,236 DEGs in ampicillin-treated cockroaches (compared to untreated cockroaches). Unlike bacterial composition, the DEGs varied between the two groups. Among major allergens, the expression of Bla g 2 decreased significantly in ampicillin-treated cockroaches (compared to untreated group).

Conclusions: In this study, the reduced level of allergens observed in cockroaches may be related to lower amounts of total bacteria caused by treatment with antibiotics. It is possible to make a protein extract with few bacteria for use in immunotherapy.
Files in This Item:
T202126207.pdf Download
DOI
10.1371/journal.pone.0257114
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Yeong(김주영) ORCID logo https://orcid.org/0000-0003-2456-6298
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190606
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links