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STING-driven interferon signaling triggers metabolic alterations in pancreas cancer cells visualized by [ 18 F]FLT PET imaging

Authors
 Keke Liang  ;  Evan R Abt  ;  Thuc M Le  ;  Arthur Cho  ;  Amanda M Dann  ;  Jing Cui 7  ;  Luyi Li  ;  Khalid Rashid  ;  Amanda L Creech  ;  Liu Wei  ;  Razmik Ghukasyan  ;  Ethan W Rosser  ;  Nanping Wu  ;  Giuseppe Carlucci  ;  Johannes Czernin  ;  Timothy R Donahue  ;  Caius G Radu 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.118(36) : e2105390118, 2021-09 
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN
 0027-8424 
Issue Date
2021-09
MeSH
Animals ; Cell Line, Tumor ; Dideoxynucleosides / administration & dosage* ; Female ; Fluorine Radioisotopes / administration & dosage* ; Humans ; Interferon Type I / metabolism* ; Male ; Membrane Proteins / metabolism* ; Mice ; Mice, Inbred NOD ; Pancreatic Neoplasms / metabolism* ; Pancreatic Neoplasms / pathology ; Positron-Emission Tomography / methods* ; Signal Transduction ; Xenograft Model Antitumor Assays
Keywords
PET imaging ; STING ; interferon ; nucleotide metabolism ; pancreatic cancer
Abstract
Type I interferons (IFNs) are critical effectors of emerging cancer immunotherapies designed to activate pattern recognition receptors (PRRs). A challenge in the clinical translation of these agents is the lack of noninvasive pharmacodynamic biomarkers that indicate increased intratumoral IFN signaling following PRR activation. Positron emission tomography (PET) imaging enables the visualization of tissue metabolic activity, but whether IFN signaling-induced alterations in tumor cell metabolism can be detected using PET has not been investigated. We found that IFN signaling augments pancreatic ductal adenocarcinoma (PDAC) cell nucleotide metabolism via transcriptional induction of metabolism-associated genes including thymidine phosphorylase (TYMP). TYMP catalyzes the first step in the catabolism of thymidine, which competitively inhibits intratumoral accumulation of the nucleoside analog PET probe 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT). Accordingly, IFN treatment up-regulates cancer cell [18F]FLT uptake in the presence of thymidine, and this effect is dependent upon TYMP expression. In vivo, genetic activation of stimulator of interferon genes (STING), a PRR highly expressed in PDAC, enhances the [18F]FLT avidity of xenograft tumors. Additionally, small molecule STING agonists trigger IFN signaling-dependent TYMP expression in PDAC cells and increase tumor [18F]FLT uptake in vivo following systemic treatment. These findings indicate that [18F]FLT accumulation in tumors is sensitive to IFN signaling and that [18F]FLT PET may serve as a pharmacodynamic biomarker for STING agonist-based therapies in PDAC and possibly other malignancies characterized by elevated STING expression.
Files in This Item:
T202126133.pdf Download
DOI
10.1073/pnas.2105390118
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
Yonsei Authors
Cho, Arthur Eung Hyuck(조응혁) ORCID logo https://orcid.org/0000-0001-8670-2473
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190516
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