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Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii

Authors
 Thao Nguyen Vu  ;  Jung Hyun Byun  ;  Roshan D'Souza  ;  Naina Adren Pinto  ;  Le Phuong Nguyen  ;  Dongeun Yong  ;  Yunsop Chong 
Citation
 ANNALS OF LABORATORY MEDICINE, Vol.40(1) : 21-26, 2020-01 
Journal Title
ANNALS OF LABORATORY MEDICINE
ISSN
 2234-3806 
Issue Date
2020-01
MeSH
Acinetobacter baumannii / drug effects ; Acinetobacter baumannii / isolation & purification ; Acinetobacter baumannii / metabolism* ; Anti-Bacterial Agents / metabolism* ; Anti-Bacterial Agents / pharmacology ; Bacterial Proteins / genetics ; Bacterial Proteins / metabolism* ; Disk Diffusion Antimicrobial Tests / methods* ; Drug Resistance, Bacterial / drug effects ; Meropenem / metabolism* ; Meropenem / pharmacology ; beta-Lactamases / genetics ; beta-Lactamases / metabolism*
Keywords
Carbapenemase ; Acinetobacter baumannii ; Modified carbapenem inactivation method ; CIMTris
Abstract
Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.
Files in This Item:
T9992020532.pdf Download
DOI
10.3343/alm.2020.40.1.21
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Byun, Jung-Hyun(변정현)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190307
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