Cited 10 times in
IL-17C Protects Nasal Epithelium from Pseudomonas aeruginosa Infection
DC Field | Value | Language |
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dc.contributor.author | 윤상선 | - |
dc.contributor.author | 이강무 | - |
dc.contributor.author | 최재영 | - |
dc.date.accessioned | 2022-09-06T06:43:15Z | - |
dc.date.available | 2022-09-06T06:43:15Z | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 1044-1549 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190290 | - |
dc.description.abstract | IL-17 family cytokines are directly involved in host immune responses and the critical mediators for host defense against infection or inflammation. IL-17C is highly expressed in respiratory epithelium and is induced after acute bacterial lung infection. However, the definite function of IL-17C induced by Pseudomonas aeruginosa (PAO1 strain) is not fully understood, and our study was designed to demonstrate IL-17C-induced immune response against PAO1 infection in nasal epithelium. Passage-2 normal human nasal epithelial (NHNE) cells were infected with PAO1 and the relationship between IL-17C-related immune responses and the iron absorption of PAO1, depending on inoculation of recombinant human IL-17C (rhIL-17C), was assessed by measuring the siderophore activity of PAO1. Microarray data showed that IL-17C expression increased 34.7 times at 8 hours postinfection (hpi) in NHNE cells, and IL-17C mRNA levels increased until 48 hpi. The PAO1 colonies significantly increased from 8 hpi in NHNE cells, and siderophore activity of PAO1 was enhanced in the supernatants of PAO1-infected NHNE cells. Interestingly, PAO1 colonies were reduced in PAO1-infected NHNE cells treated with rhIL-17C, and supernatants from NHNE cells treated with rhIL-17C also exhibited decreased PAO1 colonies. We found that the siderophore activity of PAO1 was significantly reduced in the supernatants of NHNE cells treated with rhIL-17C where LCN2 expression was highly elevated. Our findings indicate that IL-17C mediates an antibacterial effect against PAO1 by inhibiting siderophore activity in nasal epithelium. We propose that IL-17C might be an efficient mediator to suppress PAO1 infection through disturbing iron absorption of PAO1 in nasal epithelium. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Thoracic Society | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Epithelial Cells / immunology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-17 / immunology* | - |
dc.subject.MESH | Nasal Mucosa / immunology* | - |
dc.subject.MESH | Pseudomonas Infections / immunology* | - |
dc.subject.MESH | Pseudomonas aeruginosa / immunology* | - |
dc.subject.MESH | RNA, Messenger / immunology | - |
dc.subject.MESH | Respiratory Mucosa / immunology* | - |
dc.subject.MESH | Siderophores / immunology | - |
dc.title | IL-17C Protects Nasal Epithelium from Pseudomonas aeruginosa Infection | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Yung Jin Jeon | - |
dc.contributor.googleauthor | Ara Jo | - |
dc.contributor.googleauthor | Jina Won | - |
dc.contributor.googleauthor | Kang-Mu Lee | - |
dc.contributor.googleauthor | Sang Sun Yoon | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.contributor.googleauthor | Hyun Jik Kim | - |
dc.identifier.doi | 10.1165/rcmb.2018-0377OC | - |
dc.contributor.localId | A02558 | - |
dc.contributor.localId | A02638 | - |
dc.contributor.localId | A04173 | - |
dc.relation.journalcode | J00113 | - |
dc.identifier.eissn | 1535-4989 | - |
dc.identifier.pmid | 31318581 | - |
dc.identifier.url | https://www.atsjournals.org/doi/10.1165/rcmb.2018-0377OC | - |
dc.subject.keyword | Pseudomonas aeruginosa | - |
dc.subject.keyword | IL-17C | - |
dc.subject.keyword | siderophore | - |
dc.subject.keyword | iron sequestration | - |
dc.subject.keyword | nasal epithelium | - |
dc.contributor.alternativeName | Yoon, Sang Sun | - |
dc.contributor.affiliatedAuthor | 윤상선 | - |
dc.contributor.affiliatedAuthor | 이강무 | - |
dc.contributor.affiliatedAuthor | 최재영 | - |
dc.citation.volume | 62 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 95 | - |
dc.citation.endPage | 103 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, Vol.62(1) : 95-103, 2020-01 | - |
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