7 33

Cited 0 times in

Characterization of human cardiac mesenchymal stromal cells and their extracellular vesicles comparing with human bone marrow derived mesenchymal stem cells

 In Sook Kang  ;  Joowon Suh  ;  Mi-Ni Lee  ;  Chaeyoung Lee  ;  Jing Jin  ;  Changjin Lee  ;  Young Il Yang  ;  Yangsoo Jang  ;  Goo Taeg Oh 
 BMB REPORTS, Vol.53(2) : 118-123, 2020-02 
Journal Title
Issue Date
Adult Stem Cells / cytology ; Adult Stem Cells / metabolism ; Bone Marrow Cells / cytology* ; Bone Marrow Cells / metabolism ; Cell Hypoxia ; Cell Proliferation ; Cells, Cultured ; Extracellular Vesicles / metabolism* ; Extracellular Vesicles / ultrastructure ; Heart / physiology ; Human Umbilical Vein Endothelial Cells ; Humans ; Mesenchymal Stem Cells / cytology* ; Mesenchymal Stem Cells / metabolism ; Myocardium / cytology* ; Myocardium / metabolism ; Myocardium / pathology ; Neovascularization, Physiologic ; Organ Culture Techniques ; Regeneration
Cardiovascular disease ; Extracellular vesicles ; Mesenchymal stem cell ; Regeneration
Cardiac regeneration with adult stem-cell (ASC) therapy is a promising field to address advanced cardiovascular diseases. In addition, extracellular vesicles (EVs) from ASCs have been implicated in acting as paracrine factors to improve cardiac functions in ASC therapy. In our work, we isolated human cardiac mesenchymal stromal cells (h-CMSCs) by means of three-dimensional organ culture (3D culture) during ex vivo expansion of cardiac tissue, to compare the functional efficacy with human bone-marrow derived mesenchymal stem cells (h-BM-MSCs), one of the actively studied ASCs. We characterized the h-CMSCs as CD90(low), c-kit(negative), CD105(positive) phenotype and these cells express NANOG, SOX2, and GATA4. To identify the more effective type of EVs for angiogenesis among the different sources of ASCs, we isolated EVs which were derived from CMSCs with either nomioxic or hypoxic condition and BM-MSCs. Our in vitro tube-formation results demonstrated that the angiogenic effects of EVs from hypoxia-treated CMSCs (CMSC-Hpx EVs) were greater than the well-known effects of EVs from BM-MSCs (BM-MSC EVs), and these were even comparable to human vascular endothelial growth factor (hVEGF), a potent angiogenic factor. Therefore, we present here that CD90(low)c-kit(negative)CD105(positive) CMSCs under hypoxic conditions secrete functionally superior EVs for in vitro angiogenesis. Our findings will allow more insights understanding myocardial repair.
Files in This Item:
T9992020487.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.