Cited 6 times in
Assessment of clinical performance of an ultrasensitive nanowire assay for detecting human papillomavirus DNA in urine
DC Field | Value | Language |
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dc.date.accessioned | 2022-09-06T06:08:38Z | - |
dc.date.available | 2022-09-06T06:08:38Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 0090-8258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190204 | - |
dc.description.abstract | Objective. To evaluate whether HPV DNA in urine has potential advantages as an alternative biomarker for HPV-based cervical cancer screening. Methods. Among patients with Cobas HPV test results, a total of 67 HPV-positive (n = 42) and-negative (n = 25) women who agreed to participate in this study were willing to provide paired cervical and urine samples, and we observed concordance between sample types from each patient in identifying HPV genotypes using the nanowire assay. Results. We detected high-risk strains of HPV DNA in unprocessed urine specimens using polyethyleneimineconjugated nanowires (PEI-NW5). Concordance for high-risk HPV (hrHPV) between paired urine and cervical samples was 90.4% (K = 0.90; 95% CI: 0.80-100.00). The virological sensitivity and specificity for detection of HPV DNA from a small urine sample (2001.1) were 81.3% (kappa = 0.83; 95% CI: 62.1-100.0) and 98.0% (kappa = 0.83; 95% CI: 94.2-100.0) for HPVI6 group, 100.0% (kappa = 0.65; 95% CI: 100.0-100.0) and 95.3% (kappa = 0.65; 95% CI: 90.1-100.0) for HPV18 group, and 96.4% (kappa = 0.97; 95% CI: 89.6-100.0) and 100.0% (kappa = 0.97; 95% CI: 100.0-100.0) for other hrHPV group, respectively. Conclusions. The nanowire assay demonstrated excellent ability to identify HPV DNA from urine specimens. We observed an excellent agreement in the detection of high-risk HPV between paired urine and cervical samples, even with small urine sample volume. (C) 2019 Elsevier Inc. All rights reserved. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.relation.isPartOf | GYNECOLOGIC ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Biomarkers, Tumor / genetics | - |
dc.subject.MESH | Biomarkers, Tumor / urine | - |
dc.subject.MESH | Cell-Free Nucleic Acids / urine | - |
dc.subject.MESH | Cytodiagnosis / instrumentation | - |
dc.subject.MESH | Cytodiagnosis / methods | - |
dc.subject.MESH | DNA, Viral / genetics | - |
dc.subject.MESH | DNA, Viral / urine* | - |
dc.subject.MESH | Early Detection of Cancer / methods | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Human papillomavirus 16 / genetics | - |
dc.subject.MESH | Human papillomavirus 16 / isolation & purification | - |
dc.subject.MESH | Human papillomavirus 18 / genetics | - |
dc.subject.MESH | Human papillomavirus 18 / isolation & purification | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Nanowires | - |
dc.subject.MESH | Papillomaviridae / genetics* | - |
dc.subject.MESH | Papillomaviridae / isolation & purification | - |
dc.subject.MESH | Papillomavirus Infections / urine | - |
dc.subject.MESH | Papillomavirus Infections / virology* | - |
dc.subject.MESH | Polyethyleneimine | - |
dc.subject.MESH | Spectrophotometry, Ultraviolet | - |
dc.subject.MESH | Uterine Cervical Neoplasms / urine | - |
dc.subject.MESH | Uterine Cervical Neoplasms / virology* | - |
dc.title | Assessment of clinical performance of an ultrasensitive nanowire assay for detecting human papillomavirus DNA in urine | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Others | - |
dc.contributor.googleauthor | HyungJae Lee | - |
dc.contributor.googleauthor | Mihye Choi | - |
dc.contributor.googleauthor | Minkyung Jo | - |
dc.contributor.googleauthor | Eun Young Park | - |
dc.contributor.googleauthor | Sang-Hyun Hwang | - |
dc.contributor.googleauthor | Youngnam Cho | - |
dc.identifier.doi | 10.1016/j.ygyno.2019.11.031 | - |
dc.relation.journalcode | J00956 | - |
dc.identifier.eissn | 1095-6859 | - |
dc.identifier.pmid | 31926639 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0090825819316828 | - |
dc.subject.keyword | Urine | - |
dc.subject.keyword | Cell-free DNA | - |
dc.subject.keyword | HPV | - |
dc.subject.keyword | Cervical cancer | - |
dc.subject.keyword | Nanowires | - |
dc.subject.keyword | Colorimetric detection | - |
dc.citation.volume | 156 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 641 | - |
dc.citation.endPage | 646 | - |
dc.identifier.bibliographicCitation | GYNECOLOGIC ONCOLOGY, Vol.156(3) : 641-646, 2020-03 | - |
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