Cited 18 times in
Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies
DC Field | Value | Language |
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dc.contributor.author | 김철훈 | - |
dc.date.accessioned | 2022-09-06T06:05:09Z | - |
dc.date.available | 2022-09-06T06:05:09Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190153 | - |
dc.description.abstract | In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we report multiplex protein imaging with secondary ion mass spectrometry (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies with <300 nm spatial resolution in the low ion dose without ion beam damage because of the high secondary ion yields of the MONPs, which can provide simultaneous imaging of several proteins, especially from cell membranes. We applied our new imaging technique for the study of hippocampal tissue samples from control and Alzheimer's disease (AD) model mice; the proximity of protein clusters in the hippocampus CA1 region showed intriguing dependence on aging and AD progress, suggesting that protein cluster proximity may be helpful for understanding pathological pathways in the microscopic cellular level. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS APPLIED MATERIALS & INTERFACES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Alzheimer Disease / diagnostic imaging | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies / chemistry | - |
dc.subject.MESH | Antibodies / immunology* | - |
dc.subject.MESH | Antibodies, Immobilized / chemistry | - |
dc.subject.MESH | Antibodies, Immobilized / immunology | - |
dc.subject.MESH | CA1 Region, Hippocampal / diagnostic imaging | - |
dc.subject.MESH | CA1 Region, Hippocampal / metabolism | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Metal Nanoparticles / chemistry* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Oxides | - |
dc.subject.MESH | Particle Size | - |
dc.subject.MESH | Proteins / immunology* | - |
dc.subject.MESH | Proteins / metabolism | - |
dc.subject.MESH | Spectrometry, Mass, Secondary Ion / methods* | - |
dc.title | Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Dae Won Moon | - |
dc.contributor.googleauthor | Young Ho Park | - |
dc.contributor.googleauthor | Sun Young Lee | - |
dc.contributor.googleauthor | Heejin Lim | - |
dc.contributor.googleauthor | SuHwa Kwak | - |
dc.contributor.googleauthor | Minseok S Kim | - |
dc.contributor.googleauthor | Hyunmin Kim | - |
dc.contributor.googleauthor | Eunjoo Kim | - |
dc.contributor.googleauthor | Yebin Jung Hyang-Sook Hoe | - |
dc.contributor.googleauthor | Sungjee Kim | - |
dc.contributor.googleauthor | Dong-Kwon Lim | - |
dc.contributor.googleauthor | Chul-Hoon Kim | - |
dc.contributor.googleauthor | Su-Il In | - |
dc.identifier.doi | 10.1021/acsami.9b21800 | - |
dc.contributor.localId | A01057 | - |
dc.relation.journalcode | J00004 | - |
dc.identifier.eissn | 1944-8252 | - |
dc.identifier.pmid | 32073828 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsami.9b21800 | - |
dc.subject.keyword | secondary ion mass spectrometry | - |
dc.subject.keyword | multiplex protein imaging | - |
dc.subject.keyword | metal oxide nanoparticle | - |
dc.subject.keyword | Alzheimer's disease | - |
dc.subject.keyword | protein cluster proximity analysis | - |
dc.contributor.alternativeName | Kim, Chul Hoon | - |
dc.contributor.affiliatedAuthor | 김철훈 | - |
dc.citation.volume | 12 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 18056 | - |
dc.citation.endPage | 18064 | - |
dc.identifier.bibliographicCitation | ACS APPLIED MATERIALS & INTERFACES, Vol.12(15) : 18056-18064, 2020-04 | - |
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