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Surface Tethering of Inflammation-Modulatory Nanostimulators to Stem Cells for Ischemic Muscle Repair

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dc.date.accessioned2022-09-02T01:16:32Z-
dc.date.available2022-09-02T01:16:32Z-
dc.date.issued2020-05-
dc.identifier.issn1936-0851-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/190112-
dc.description.abstractStem cell transplantation has been a promising treatment for peripheral arterial diseases in the past decade. Stem cells act as living bioreactors of paracrine factors that orchestrate tissue regeneration. Prestimulated adipose-derived stem cells (ADSCs) have been proposed as potential candidates but have been met with challenges in activating their secretory activities for clinical use. Here, we propose that tethering the ADSC surface with nanoparticles releasing tumor necrosis factor alpha (TNF alpha), named nanostimulator, would stimulate cellular secretory activity in situ. We examined this hypothesis by complexing octadecylamine-grafted hyaluronic acid onto a liposomal carrier of TNF alpha. Hyaluronic acid increased the liposomal stability and association to CD44 on ADSC surface. ADSCs tethered with these TNF alpha carriers exhibited up-regulated secretion of proangiogenic vascular endothelial growth factor and immunomodulatory prosteoglandin E2 (PGE(2)) while decreasing secretion of antiangiogenic pigment epithelium-derived factors. Accordingly, ADSCs tethered with nanostimulators promoted vascularization in a 3D microvascular chip and enhanced recovery of perfusion, walking, and muscle mass in a murine ischemic hindlimb compared to untreated ADSCs. We propose that this surface tethering strategy for in situ stimulation of stem cells would replace the costly and cumbersome preconditioning process and expedite clinical use of stem cells for improved treatments of various injuries and diseases.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAmerican Chemical Society-
dc.relation.isPartOfACS NANO-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAdipose Tissue-
dc.subject.MESHAnimals-
dc.subject.MESHCells, Cultured-
dc.subject.MESHInflammation-
dc.subject.MESHMice-
dc.subject.MESHMuscles-
dc.subject.MESHStem Cell Transplantation-
dc.subject.MESHStem Cells*-
dc.subject.MESHVascular Endothelial Growth Factor A*-
dc.titleSurface Tethering of Inflammation-Modulatory Nanostimulators to Stem Cells for Ischemic Muscle Repair-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorJiayu Leong-
dc.contributor.googleauthorYu-Tong Hong-
dc.contributor.googleauthorYu-Fu Wu-
dc.contributor.googleauthorEunkyung Ko-
dc.contributor.googleauthorSvyatoslav Dvoretskiy-
dc.contributor.googleauthorJye Yng Teo-
dc.contributor.googleauthorByoung Soo Kim-
dc.contributor.googleauthorKyeongsoo Kim-
dc.contributor.googleauthorHojeong Jeon-
dc.contributor.googleauthorMarni Boppart-
dc.contributor.googleauthorYi Yan Yang-
dc.contributor.googleauthorHyunjoon Kong-
dc.identifier.doi10.1021/acsnano.9b04926-
dc.relation.journalcodeJ00005-
dc.identifier.eissn1936-086X-
dc.identifier.pmid32243129-
dc.subject.keywordangiogenesis-
dc.subject.keywordadipose-derived stem cells-
dc.subject.keywordhyaluronic acid-
dc.subject.keywordvascular endothelial growth factor-
dc.subject.keywordmuscle-
dc.subject.keywordliposome-
dc.citation.volume14-
dc.citation.number5-
dc.citation.startPage5298-
dc.citation.endPage5313-
dc.identifier.bibliographicCitationACS NANO, Vol.14(5) : 5298-5313, 2020-05-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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