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Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells

 Ji-Hun Shin  ;  Jung-Pyo Yang  ;  Seung-Hwan Seo  ;  Sang-Gyun Kim  ;  Eun-Min Kim  ;  Do-Won Ham  ;  Eun-Hee Shin 
 BMB REPORTS, Vol.53(9) : 478-483, 2020-09 
Journal Title
Issue Date
Animals ; Cytokines / analysis ; Cytokines / immunology ; Flounder / parasitology ; HT29 Cells ; Humans ; Lectins, C-Type / immunology* ; Myxozoa / genetics ; Myxozoa / immunology* ; Parasitic Diseases / immunology* ; Parasitic Diseases / parasitology ; Spores / immunology
Cell adhesion ; C-type lectin Mincle ; Innate immunity ; Myxozoan parasite ; Signal transduction
Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudoa spores were isolated from olive flounder, HT29 cells were exposed to spores identified to be alive using SYTO-9 and propidium iodide staining or to antigens of Kudoa spores (KsAg). IL-1 beta, IL-8, TNF-alpha and NFKB1 expression and NF-kappa B activation were assessed using real-time PCR, cytokine array and western blotting. The immunofluorescence of FITC-conjugated lectins, results of ligand binding assays using Mincle-Fc and IgG-Fc, CLEC4E expressions in response to KsAg stimulation, and Mincle-dependent NF-kappa B activation were assessed to clarify the early immune-triggering mechanism. Inflammatory cytokines (IL-1 beta, GM-CSF and TNF-alpha), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-.B activation (pNF-kappa B/NF-kappa B) in HT29 cells increased following stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglutinin) suggested the importance of Mincle in molecular recognition between Kudoa spores and intestinal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA expression, Mincle immunofluorescence staining and hMincle-dependent NF-kappa B activation demonstrated the involvement of Mincle in the early immune-triggering mechanism. The present study newly elucidated that the molecular recognition and immune-triggering mechanism of K. septempunctata are associated with Mincle on human intestinal epithelial cells.
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1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun Min(김은민)
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