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Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure

Authors
 Ji Young Kim  ;  Eun Jung Lee  ;  Jimyung Seo  ;  Yangsin Lee  ;  Yuri Ahn  ;  Sujin Park  ;  Yu Jeong Bae  ;  Jinu Lee  ;  Beom Jin Lim  ;  Doyoung Kim  ;  Jin Won Cho  ;  Sang Ho Oh 
Citation
 FASEB JOURNAL, Vol.36(7) : e22424, 2022-07 
Journal Title
FASEB JOURNAL
ISSN
 0892-6638 
Issue Date
2022-07
MeSH
Animals ; Cell Movement / physiology ; Epidermis* / metabolism ; Glucose / metabolism ; Humans ; Keratinocytes* / metabolism ; Membrane Proteins / genetics ; Membrane Proteins / metabolism ; Mice
Keywords
cell migration ; keratinocytes ; nephrin ; wound healing ; zonula occludens-1
Abstract
Nephrin is a type-1 transmembrane protein and a component of the slit diaphragm renal-filtration barrier. It has several functions in actin remodeling and cell-cell adhesion. Nephrin is principally located in the kidney glomerulus, but several studies have reported that nephrin is found in the pancreas, brain, and placenta. However, nephrin expression and its role in human skin have not yet been reported. First, using single-cell RNA sequencing, immunohistochemistry, and immuno-electron microscopy, nephrin expression was confirmed in human-skin epidermal keratinocytes. Nephrin expression colocalized with the expression of zonula occludens-1 in keratinocytes and was closely related to keratinocyte cell density, proliferation, and migration. High glucose treatment decreased nephrin expression and compromised keratinocyte cell migration without yes-associated protein nuclear entry. This reduced cell migration under high glucose conditions was improved in nephrin-overexpressing keratinocytes. Nephrin was highly expressed on the margins of re-epithelized epidermis based on in vivo mice and ex vivo human skin wound models. The results demonstrate that nephrin is expressed in human-skin keratinocytes and functions in cell adhesion, proliferation, and migration. In conclusion, this study suggests that nephrin may have a variety of physiological roles in human skin.
Full Text
https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202100455RR
DOI
10.1096/fj.202100455RR
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영) ORCID logo https://orcid.org/0000-0002-0194-9854
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lim, Beom Jin(임범진) ORCID logo https://orcid.org/0000-0003-2856-0133
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189583
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