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Parasites and blood-meal hosts of the tsetse fly in Tanzania: a metagenomics study

Authors
 Ju Yeong Kim  ;  Jun Ho Choi  ;  Sung-Hyun Nam  ;  Robert Fyumagwa  ;  Tai-Soon Yong 
Citation
 PARASITES & VECTORS, Vol.15(1) : 224, 2022-06 
Journal Title
PARASITES & VECTORS
Issue Date
2022-06
MeSH
Animals ; Animals, Wild / parasitology ; Insect Vectors / parasitology ; Mammals / genetics ; Metagenomics ; Mice ; Parasites* / genetics ; RNA, Ribosomal, 18S / genetics ; Swine ; Tanzania ; Trypanosoma* / genetics ; Trypanosomiasis, African* ; Tsetse Flies* / parasitology
Keywords
Amplicon deep sequencing ; Tanzania ; Trypanosoma ; Trypanosomiasis ; Tsetse fly
Abstract
Background: Tsetse flies can transmit various Trypanosoma spp. that cause trypanosomiasis in humans, wild animals, and domestic animals. Amplicon deep sequencing of the 12S ribosomal RNA (rRNA) gene can be used to detect mammalian tsetse hosts, and the 18S rRNA gene can be used to detect all associated eukaryotic pathogens, including Trypanosoma spp.

Methods: Tsetse flies were collected from the Serengeti National Park (n = 48), Maswa Game Reserve (n = 42), and Tarangire National Park (n = 49) in Tanzania in 2012-13. Amplicon deep sequencing targeting mammal-specific 12S rRNA and 18S rRNA genes was performed to screen the blood-feeding sources of tsetse flies and eukaryotic parasites in tsetse flies, respectively.

Results: 12S rRNA gene deep sequencing revealed that various mammals were blood-feeding sources of the tsetse flies, including humans, common warthogs, African buffalos, mice, giraffes, African elephants, waterbucks, and lions. Genes of humans were less frequently detected in Serengeti (P = 0.0024), whereas African buffaloes were detected more frequently as a blood-feeding source (P = 0.0010). 18S rRNA gene deep sequencing showed that six tsetse samples harbored the Trypanosoma gene, which was identified as Trypanosoma godfreyi and Trypanosoma simiae in subsequent ITS1 gene sequencing.

Conclusions: Through amplicon deep sequencing targeting the 12S rRNA and 18S rRNA genes, various mammalian animals were identified as blood-meal sources, and two Trypanosoma species were detected in tsetse flies collected from the Maswa Game Reserve, Serengeti National Park, and Tarangire National Park in Tanzania. This study illustrates the patterns of parasitism of tsetse fly, wild animals targeted by the fly, and Trypanosoma spp. carried by the fly in Tanzania. It may provide essential data for formulating better strategies to control African trypanosomes.
Files in This Item:
T202202891.pdf Download
DOI
10.1186/s13071-022-05344-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Young(김주영) ORCID logo https://orcid.org/0000-0003-2456-6298
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Choi, Jun Ho(최준호) ORCID logo https://orcid.org/0000-0002-7416-3377
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189528
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