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Inflammatory Monocytes Infiltrate the Spiral Ligament and Migrate to the Basilar Membrane After Noise Exposure

Authors
 Seung Ho Shin  ;  Jee Eun Yoo  ;  Jinsei Jung  ;  Jae Young Choi  ;  Seong Hoon Bae 
Citation
 CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, Vol.15(2) : 153-159, 2022-05 
Journal Title
CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
ISSN
 1976-8710 
Issue Date
2022-05
Keywords
Cochlea ; Macrophages ; Monocytes ; Noise
Abstract
Objectives: Noise exposure leads to an increase in the macrophage population. This increment is thought to be caused by the transformation of infiltrated monocytes into macrophages rather than by proliferation of the cochlear resident macrophages. However, studies on infiltrated monocytes in the cochlea are scarce. Thus, we aimed to investigate the infiltration of monocytes and their transformation into macrophages after noise exposure.

Methods: In wild-type and CX3CR1+/GFP C57/B6 mice, inflammatory monocytes were identified by immunofluorescence of mouse cochlear cells. The findings were confirmed and quantitated by flow cytometry.

Results: One day after noise exposure, monocytes were identified in the spiral ligament. Flow cytometric analysis confirmed that the monocyte population peaked on post-noise exposure day 1 and decreased thereafter. On day 3 after noise exposure, amoeboid-type macrophages increased in the crista basilaris, and on day 5, they spread to the basilar membrane.

Conclusion: Infiltrated monocytes were successfully observed 1 day after noise exposure, preceding the increase in the macrophage population. This finding supports the proposal that infiltrated monocytes transform into macrophages.
Files in This Item:
T202202424.pdf Download
DOI
10.21053/ceo.2021.00857
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Bae, Seong Hoon(배성훈) ORCID logo https://orcid.org/0000-0001-9243-9392
Shin, Seung Ho(신승호) ORCID logo https://orcid.org/0000-0001-9352-8426
Jung, Jinsei(정진세) ORCID logo https://orcid.org/0000-0003-1906-6969
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189418
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