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Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands

Authors
 Yeo-Jun Yoon  ;  Donghyun Kim  ;  Kwon Yong Tak  ;  Seungyeon Hwang  ;  Jisun Kim  ;  Nam Suk Sim  ;  Jae-Min Cho  ;  Dojin Choi  ;  Yongmi Ji  ;  Junho K Hur  ;  Hyunki Kim  ;  Jong-Eun Park  ;  Jae-Yol Lim 
Citation
 NATURE COMMUNICATIONS, Vol.13(1) : 3291, 2022-06 
Journal Title
NATURE COMMUNICATIONS
Issue Date
2022-06
MeSH
Animals ; Humans ; Mice ; Organoids* / metabolism ; Saliva / metabolism ; Salivary Gland Neoplasms* / metabolism ; Salivary Glands
Abstract
Salivary glands that produce and secrete saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we establish long-term murine and human salivary gland organoid cultures. Murine and human salivary gland organoids express gland-specific genes and proteins of acinar, myoepithelial, and duct cells, and exhibit gland functions when stimulated with neurotransmitters. Furthermore, human salivary gland organoids are established from isolated basal or luminal cells, retaining their characteristics. Single-cell RNA sequencing also indicates that human salivary gland organoids contain heterogeneous cell types and replicate glandular diversity. Our protocol also enables the generation of tumoroid cultures from benign and malignant salivary gland tumor types, in which tumor-specific gene signatures are well-conserved. In this study, we provide an experimental platform for the exploration of precision medicine in the era of tissue regeneration and anticancer treatment.
Files in This Item:
T202202373.pdf Download
DOI
10.1038/s41467-022-30934-z
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Donghyun(김동현) ORCID logo https://orcid.org/0000-0001-7863-7384
Kim, Hyunki(김현기) ORCID logo https://orcid.org/0000-0003-2292-5584
Sim, Nam Suk(심남석)
Yoon, Y J(윤여준)
Lim, Jae Yol(임재열) ORCID logo https://orcid.org/0000-0002-9757-6414
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189395
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