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EGFR-targeted fluorescent imaging using the da Vinci® Firefly™ camera for gallbladder cancer

Authors
 Jung Ha Choi  ;  Chang Moo Kang  ;  Jeong Youp Park 
Citation
 WORLD JOURNAL OF SURGICAL ONCOLOGY, Vol.20(1) : 201, 2022-06 
Journal Title
WORLD JOURNAL OF SURGICAL ONCOLOGY
Issue Date
2022-06
MeSH
Animals ; Cell Line, Tumor ; Disease Models, Animal ; ErbB Receptors ; Fluorescent Dyes / chemistry ; Gallbladder Neoplasms* / diagnostic imaging ; Gallbladder Neoplasms* / pathology ; Gallbladder Neoplasms* / surgery ; HEK293 Cells ; Humans ; Mice ; Mice, Nude
Keywords
EGFR ; Gallbladder cancer ; Imaging ; Robotic surgery
Abstract
Background: Fluorescent imaging may aid with the precise diagnosis and treatment of patients with gallbladder cancer. In this study, we sought to demonstrate whether the da Vinci® surgical system and Firefly™ camera could detect EGFR-targeted fluorescent images in orthotopic mouse models of gallbladder cancer.

Methods: An orthotopic mouse model of gallbladder cancer was created by injecting NOZ gallbladder cancer cells mixed with Matrigel into the gallbladder. In vivo imaging of subcutaneous and orthotopic gallbladder tumors was performed after the injection of DyLight 650- or 800-conjugated EGFR antibody.

Results: Western blotting, flow cytometry, and confocal microscopy showed the presence of EGFR in NOZ cells, but not in HEK293 cells. Subcutaneous NOZ cell tumors fluoresced after injection with fluorescent EGFR antibody, but subcutaneous HEK293 tumors did not. Fluorescent EGFR antibody made orthotopic NOZ tumors fluoresce, with an intensity stronger than that in the surrounding normal tissues. Histochemical examination confirmed the location of the tumors inside the gallbladder and adjacent liver parenchyma. Fluorescent signal was also detected in orthotopic gallbladder tumors with Firefly™ camera.

Conclusion: Our study showed that fluorescent EGFR antibodies and the Firefly camera in the da Vinci system can detect fluorescing gallbladder tumors, which demonstrates their potential use for molecular imaging-based prevision surgery in the near future.
Files in This Item:
T202202198.pdf Download
DOI
10.1186/s12957-022-02675-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chang Moo(강창무) ORCID logo https://orcid.org/0000-0002-5382-4658
Park, Jeong Youp(박정엽) ORCID logo https://orcid.org/0000-0003-0110-8606
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189305
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