Cited 5 times in
PANCREATITIS-ASSOCIATED PROTEIN-1 SUPPRESSES APOPTOSIS IN CERULEIN-STIMULATED PANCREATIC ACINAR CELLS IN RESPONSE TO NUCLEAR FACTOR-KAPPA B ACTIVATION
DC Field | Value | Language |
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dc.date.accessioned | 2022-08-19T06:27:03Z | - |
dc.date.available | 2022-08-19T06:27:03Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 0867-5910 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/189183 | - |
dc.description.abstract | Pancreatitis is a disease for which there are numerous etiologies but no effective treatments. Although the expression of the pancreatitis-associated protein-1 (PAP-1) serves as a marker for the disease, its biological function is unknown. The present study was carried out to determine if PAP-1 performs a protective role against oxidative stress-induced pancreatic cell death. For this purpose, we used cerulein-stimulated pancreatic acinar AR42J cells as an experimental model of acute pancreatitis. First, we demonstrated that PAP-1 gene expression is increased by cerulein in a dose- and time-dependent manner. In parallel, the level of active nuclear factor kappaB (NF-kappa B) was found to be increased in cells treated with cerulein. To test whether activation of the oxidant-sensitive transcription factor NF-kappa B is mediated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, the primary source of reactive oxygen species, cerulein-stimulated NADPH oxidase activity was suppressed by using the NADPH oxidase inhibitor diphenyleneiodonium and, separately, by anti-sense oligonucleotides directed against NADPH oxidase subunits p22(phox) and p47(phox). We observed that a decrease in NADPH oxidase activity resulted in decreased NF-kappa B activation and decreased PAP-1 gene expression. To determine whether the cerulein-induced NF-kappa B activation involves PAP-1 expression, cells were transfected to overexpress the MAD3 double-point I kappa B alpha mutant. In response, NF-kappa B activation and PAP-1 gene expression were decreased. Lastly, we observed that the cerulein-induced reduction in cell viability and increase in apoptosis are reversed by overexpression of PAP-1 in PAP-1-transfected cells. Taken together, these results support the postulate that PAP-1 inhibits cerulein-induced apoptosis in response to NADPH oxidase-mediated NF-kappa B activation in pancreatic acinar cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Polish Physiological Society | - |
dc.relation.isPartOf | JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Acinar Cells / drug effects* | - |
dc.subject.MESH | Acinar Cells / pathology | - |
dc.subject.MESH | Acute Disease | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis / physiology | - |
dc.subject.MESH | Cell Survival / drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Ceruletide | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | NADPH Oxidases / metabolism | - |
dc.subject.MESH | NF-kappa B / metabolism | - |
dc.subject.MESH | Oxidative Stress | - |
dc.subject.MESH | Pancreatitis / physiopathology* | - |
dc.subject.MESH | Pancreatitis-Associated Proteins / genetics* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species / metabolism | - |
dc.title | PANCREATITIS-ASSOCIATED PROTEIN-1 SUPPRESSES APOPTOSIS IN CERULEIN-STIMULATED PANCREATIC ACINAR CELLS IN RESPONSE TO NUCLEAR FACTOR-KAPPA B ACTIVATION | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | J H Yu | - |
dc.contributor.googleauthor | J W Lim | - |
dc.contributor.googleauthor | H Kim | - |
dc.identifier.doi | 10.26402/jpp.2019.6.04 | - |
dc.relation.journalcode | J01711 | - |
dc.identifier.eissn | 1899-1505 | - |
dc.identifier.pmid | 32084646 | - |
dc.subject.keyword | cerulein | - |
dc.subject.keyword | nicotinamide adenine dinucleotide phosphate oxidase | - |
dc.subject.keyword | nuclear factor-kappa B | - |
dc.subject.keyword | pancreatitis-associated protein-1 | - |
dc.subject.keyword | pancreatic acinar cells | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | reactive oxygen species | - |
dc.citation.volume | 70 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 849 | - |
dc.citation.endPage | 857 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, Vol.70(6) : 849-857, 2019-12 | - |
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