0 38

Cited 0 times in

Ethanol Extract of Ligularia fischeri Inhibits the Lipopolysaccharide-Induced Inflammatory Response by Exerting Anti-Histone Acetyltransferase Activity to Negatively Regulate p65

Authors
 Hyo-Jin Kim  ;  Hyo-Kyoung Choi  ;  Min-Yu Chung  ;  Jae-Ho Park  ;  Sangwon Chung  ;  Seung-Hyun Lee  ;  Jin-Taek Hwang 
Citation
 JOURNAL OF MEDICINAL FOOD, Vol.22(11) : 1127-1135, 2019-11 
Journal Title
JOURNAL OF MEDICINAL FOOD
ISSN
 1096-620X 
Issue Date
2019-11
MeSH
Acetylation ; Animals ; Histone Acetyltransferases / antagonists & inhibitors* ; Inflammation* ; Interleukin-1beta / metabolism ; Interleukin-6 / metabolism ; Ligularia / chemistry* ; Lipopolysaccharides ; Mice ; Plant Extracts / pharmacology* ; RAW 264.7 Cells ; Transcription Factor RelA / metabolism* ; Tumor Necrosis Factor-alpha / metabolism
Keywords
Ligularia fischeri ; Raw 264.7 cells ; histone acetyltransferase ; inflammation ; p65
Abstract
Histone acetyltransferase (HAT) activity is well established to regulate inflammatory responses. In contrast, the mechanisms by which natural nutritional extracts influence epigenetic mechanisms to regulate inflammation have not yet been thoroughly investigated. Thus, in the present study, we observed that the anti-HAT activity exerted by an ethanol extract of Ligularia fischeri (ELF) inhibited inflammation. Specifically, we used a cell-free system to show that ELF attenuates HAT activity. We also demonstrated that ELF decreases lipopolysaccharide (LPS)-induced HAT mRNA and protein expression levels in Raw 264.7 cells, and thereby attenuates inflammation-induced patterns of hyperacetylation at nonhistone and histone-H4 proteins. Interestingly, we found that ELF blocked p65 translocation in LPS-stimulated Raw 264.7 cells by attenuating acetylation at lysine residue 310 of p65. Finally, we investigated whether ELF reduces the inflammatory cytokines, IL-6, IL-1β, and TNFα, using its HAT inhibitor activity. Taken together, these results suggest that ELF negatively regulates inflammatory responses by inhibiting HATs and HAT activity.
Full Text
https://www.liebertpub.com/doi/10.1089/jmf.2019.4412
DOI
10.1089/jmf.2019.4412
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Lee, Seung Hyun(이승현) ORCID logo https://orcid.org/0000-0001-7549-9430
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188988
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links