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Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

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dc.contributor.author정한성-
dc.date.accessioned2022-08-16T01:32:45Z-
dc.date.available2022-08-16T01:32:45Z-
dc.date.issued2018-
dc.identifier.issn1341-7649-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/188934-
dc.description.abstractInduced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and these cells have the potential to differentiate into dental mesenchymal cells, which subsequently differentiate into odontoblasts and dental pulp cells. In this study, we show that iPS-NCLCs can differentiate into mesenchymal stem cells (iPS-NCLC-MSCs), which contribute to craniofacial bone regeneration. iPS-NCLCs were cultured in serum-containing media and differentiated into functional MSCs, as confirmed by expression MSC markers and their ability to differentiate into osteoblasts, adipocytes, and chondrocytes in vitro. iPS-NCLC-MSCs were negative for markers of undifferentiated iPS cells and did not develop into teratomas when transplanted to immunodeficient mice. Further, iPS-NCLC-MSCs grew normally and differentiated into osteoblasts on hydroxyapatite scaffolds in vitro. To assess the potential of iPS-NCLC-MSCs to regenerate craniofacial bone in vivo, iPS-NCLC-MSCs were transplanted into critical-size calvarial defects in immunodeficient mice for 8 weeks. Histological analysis revealed that iPS-NCLC-MSCs differentiated into osteoblasts and contributed to bone regeneration without tumor formation. These results indicate that iPS-NCLC-MSCs could be a potential candidate for cell-based craniofacial bone tissue repair and regeneration.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish, Japanese-
dc.publisherHard Tissue Biology Network Association-
dc.relation.isPartOfJOURNAL OF HARD TISSUE BIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleCraniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학교실)-
dc.contributor.googleauthorKazuko Kikuchi-
dc.contributor.googleauthorTomoyuki Masuda-
dc.contributor.googleauthorNaoki Fujiwara-
dc.contributor.googleauthorAkiyoshi Kuji-
dc.contributor.googleauthorHiroyuki Miura-
dc.contributor.googleauthorHan-Sung Jung-
dc.contributor.googleauthorHidemitsu Harada-
dc.contributor.googleauthorKeishi Otsu-
dc.identifier.doi10.2485/jhtb.27.1-
dc.contributor.localIdA03758-
dc.relation.journalcodeJ01430-
dc.identifier.eissn1880-828X-
dc.contributor.alternativeNameJung, Han Sung-
dc.contributor.affiliatedAuthor정한성-
dc.citation.volume27-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage10-
dc.identifier.bibliographicCitationJOURNAL OF HARD TISSUE BIOLOGY, Vol.27(1) : 1-10, 2018-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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