Cited 7 times in
Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells
DC Field | Value | Language |
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dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2022-08-16T01:32:45Z | - |
dc.date.available | 2022-08-16T01:32:45Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1341-7649 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/188934 | - |
dc.description.abstract | Induced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and these cells have the potential to differentiate into dental mesenchymal cells, which subsequently differentiate into odontoblasts and dental pulp cells. In this study, we show that iPS-NCLCs can differentiate into mesenchymal stem cells (iPS-NCLC-MSCs), which contribute to craniofacial bone regeneration. iPS-NCLCs were cultured in serum-containing media and differentiated into functional MSCs, as confirmed by expression MSC markers and their ability to differentiate into osteoblasts, adipocytes, and chondrocytes in vitro. iPS-NCLC-MSCs were negative for markers of undifferentiated iPS cells and did not develop into teratomas when transplanted to immunodeficient mice. Further, iPS-NCLC-MSCs grew normally and differentiated into osteoblasts on hydroxyapatite scaffolds in vitro. To assess the potential of iPS-NCLC-MSCs to regenerate craniofacial bone in vivo, iPS-NCLC-MSCs were transplanted into critical-size calvarial defects in immunodeficient mice for 8 weeks. Histological analysis revealed that iPS-NCLC-MSCs differentiated into osteoblasts and contributed to bone regeneration without tumor formation. These results indicate that iPS-NCLC-MSCs could be a potential candidate for cell-based craniofacial bone tissue repair and regeneration. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English, Japanese | - |
dc.publisher | Hard Tissue Biology Network Association | - |
dc.relation.isPartOf | JOURNAL OF HARD TISSUE BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Kazuko Kikuchi | - |
dc.contributor.googleauthor | Tomoyuki Masuda | - |
dc.contributor.googleauthor | Naoki Fujiwara | - |
dc.contributor.googleauthor | Akiyoshi Kuji | - |
dc.contributor.googleauthor | Hiroyuki Miura | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.contributor.googleauthor | Hidemitsu Harada | - |
dc.contributor.googleauthor | Keishi Otsu | - |
dc.identifier.doi | 10.2485/jhtb.27.1 | - |
dc.contributor.localId | A03758 | - |
dc.relation.journalcode | J01430 | - |
dc.identifier.eissn | 1880-828X | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | 정한성 | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.identifier.bibliographicCitation | JOURNAL OF HARD TISSUE BIOLOGY, Vol.27(1) : 1-10, 2018 | - |
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