Cited 9 times in
A Senolytic-Eluting Coronary Stent for the Prevention of In-Stent Restenosis
DC Field | Value | Language |
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dc.contributor.author | 김중선 | - |
dc.contributor.author | 이승준 | - |
dc.date.accessioned | 2022-07-08T03:09:57Z | - |
dc.date.available | 2022-07-08T03:09:57Z | - |
dc.date.issued | 2022-05 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/188699 | - |
dc.description.abstract | The vast majority of drug-eluting stents (DES) elute either sirolimus or one of its analogues. While limus drugs stymie vascular smooth muscle cell (VSMC) proliferation to prevent in-stent restenosis, their antiproliferative nature is indiscriminate and limits healing of the endothelium in stented vessels, increasing the risk of late-stent thrombosis. Oxidative stress, which is associated with vascular injury from stent implantation, can induce VSMCs to undergo senescence, and senescent VSMCs can produce pro-inflammatory cytokines capable of inducing proliferation of neighboring nonsenescent VSMCs. We explored the potential of senolytic therapy, which involves the selective elimination of senescent cells, in the form of a senolytic-eluting stent (SES) for interventional cardiology. Oxidative stress was modeled in vitro by exposing VSMCs to H2O2, and H2O2-mediated senescence was evaluated by cytochemical staining of senescence-associated β-galactosidase activity and qRT-PCR. Quiescent VSMCs were then treated with the conditioned medium (CM) of H2O2-treated VSMCs. Proliferative effects of CM were analyzed by staining for proliferating cell nuclear antigen. Senolytic effects of the first-generation senolytic ABT263 were observed in vitro, and the effects of ABT263 on endothelial cells were also investigated through an in vitro re-endothelialization assay. SESs were prepared by dip coating. Iliofemoral arteries of hypercholesteremic rabbits were implanted with SES, everolimus-eluting stents (EESs), or bare-metal stents (BMSs), and the area of stenosis was measured 4 weeks post-implantation using optical coherence tomography. We found that a portion of H2O2-treated VSMCs underwent senescence, and that CM of H2O2-treated senescent VSMCs triggered the proliferation of quiescent VSMCs. ABT263 reverted H2O2-mediated senescence and the proliferative capacity of senescent VSMC CM. Unlike everolimus, ABT263 did not affect endothelial cell migration and/or proliferation. SES, but not EES, significantly reduced stenosis area in vivo compared with bare-metal stents (BMSs). This study shows the potential of SES as an alternative to current forms of DES. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS BIOMATERIALS SCIENCE & ENGINEERING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Constriction, Pathologic | - |
dc.subject.MESH | Coronary Restenosis* / prevention & control | - |
dc.subject.MESH | Drug-Eluting Stents* / adverse effects | - |
dc.subject.MESH | Endothelial Cells | - |
dc.subject.MESH | Everolimus / pharmacology | - |
dc.subject.MESH | Hydrogen Peroxide / pharmacology | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Senotherapeutics | - |
dc.subject.MESH | Stents | - |
dc.title | A Senolytic-Eluting Coronary Stent for the Prevention of In-Stent Restenosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Cheesue Kim | - |
dc.contributor.googleauthor | Seul-Gee Lee | - |
dc.contributor.googleauthor | Songhyun Lim | - |
dc.contributor.googleauthor | Mungyo Jung | - |
dc.contributor.googleauthor | Sung Pil Kwon | - |
dc.contributor.googleauthor | Jihye Hong | - |
dc.contributor.googleauthor | Mikyung Kang | - |
dc.contributor.googleauthor | Hee Su Sohn | - |
dc.contributor.googleauthor | Seokhyeong Go | - |
dc.contributor.googleauthor | Sangjun Moon | - |
dc.contributor.googleauthor | Seung-Jun Lee | - |
dc.contributor.googleauthor | Jung-Sun Kim | - |
dc.identifier.doi | 10.1021/acsbiomaterials.1c01611 | - |
dc.contributor.localId | A00961 | - |
dc.contributor.localId | A02927 | - |
dc.relation.journalcode | J03391 | - |
dc.identifier.eissn | 2373-9878 | - |
dc.identifier.pmid | 35416659 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsbiomaterials.1c01611?cookieSet=1 | - |
dc.subject.keyword | angioplasty | - |
dc.subject.keyword | cellular senescence | - |
dc.subject.keyword | in-stent restenosis | - |
dc.subject.keyword | senolytic therapy | - |
dc.contributor.alternativeName | Kim, Jung Sun | - |
dc.contributor.affiliatedAuthor | 김중선 | - |
dc.contributor.affiliatedAuthor | 이승준 | - |
dc.citation.volume | 8 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1921 | - |
dc.citation.endPage | 1929 | - |
dc.identifier.bibliographicCitation | ACS BIOMATERIALS SCIENCE & ENGINEERING, Vol.8(5) : 1921-1929, 2022-05 | - |
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