302 530

Cited 3 times in

Comparative Analyses of Clearing Efficacies of Tissue Clearing Protocols by Using a Punching Assisted Clarity Analysis

Authors
 Jiwon Woo  ;  Eunice Yoojin Lee  ;  Mirae Lee  ;  Seockmo Ku  ;  Jeong-Yoon Park  ;  Yong Eun Cho 
Citation
 FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, Vol.9 : 784626, 2022-01 
Journal Title
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Issue Date
2022-01
Keywords
PACA-Glow ; PACA-Light ; tissue clearing technique ; tissue transparency ; transparent brains
Abstract
The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization of three-dimensional structures with unprecedented depth and detail. Although a variety of clearing protocols have been developed, little has been done to quantify their efficacies in a systematic, reproducible fashion. Here, we present two simple assays, Punching-Assisted Clarity Analysis (PACA)-Light and PACA-Glow, which use easily accessible spectroscopy and gel documentation systems to quantify the transparency of multiple cleared tissues simultaneously. We demonstrate the use of PACA-Light and PACA-Glow to compare twenty-eight tissue clearing protocols on rodent brains. We also show that regional differences exist in tissue transparency in the rodent brain, with cerebellar tissue consistently achieving lower clearing levels compared to the prefrontal or cerebral cortex across all protocols. This represents the largest comparative study of tissue clearing protocols to date, made possible by the high-throughput nature of our PACA platforms.
Files in This Item:
T202201420.pdf Download
DOI
10.3389/fbioe.2021.784626
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
Woo, Jiwon(우지원)
Cho, Yong Eun(조용은) ORCID logo https://orcid.org/0000-0001-9815-2720
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188517
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links