2 244

Cited 13 times in

Super-resolution imaging of platelet-activation process and its quantitative analysis

Authors
 Jinkyoung Chung  ;  Dokyung Jeong  ;  Geun-Ho Kim  ;  Seokran Go  ;  Jaewoo Song  ;  Eunyoung Moon  ;  Yang Hoon Huh  ;  Doory Kim 
Citation
 SCIENTIFIC REPORTS, Vol.11(1) : 10511, 2021-05 
Journal Title
SCIENTIFIC REPORTS
Issue Date
2021-05
MeSH
Actin Cytoskeleton / drug effects ; Humans ; Organelles / metabolism ; Platelet Activation / drug effects* ; Tetradecanoylphorbol Acetate / pharmacology*
Abstract
Understanding the platelet activation molecular pathways by characterizing specific protein clusters within platelets is essential to identify the platelet activation state and improve the existing therapies for hemostatic disorders. Here, we employed various state-of-the-art super-resolution imaging and quantification methods to characterize the platelet spatiotemporal ultrastructural change during the activation process due to phorbol 12-myristate 13-acetate (PMA) stimuli by observing the cytoskeletal elements and various organelles at nanoscale, which cannot be done using conventional microscopy. Platelets could be spread out with the guidance of actin and microtubules, and most organelles were centralized probably due to the limited space of the peripheral thin regions or the close association with the open canalicular system (OCS). Among the centralized organelles, we provided evidence that granules are fused with the OCS to release their cargo through enlarged OCS. These findings highlight the concerted ultrastructural reorganization and relative arrangements of various organelles upon activation and call for a reassessment of previously unresolved complex and multi-factorial activation processes.
Files in This Item:
T202125615.pdf Download
DOI
10.1038/s41598-021-89799-9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Song, Jae Woo(송재우) ORCID logo https://orcid.org/0000-0002-1877-5731
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188143
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links