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Tonsil-derived mesenchymal stem cells incorporated in reactive oxygen species-releasing hydrogel promote bone formation by increasing the translocation of cell surface GRP78

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dc.contributor.author박종철-
dc.date.accessioned2022-05-09T16:42:03Z-
dc.date.available2022-05-09T16:42:03Z-
dc.date.issued2021-11-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/188142-
dc.description.abstractControlling the senescence of mesenchymal stem cells (MSCs) is essential for improving the efficacy of MSC-based therapies. Here, a model of MSC senescence was established by replicative subculture in tonsil-derived MSCs (TMSCs) using senescence-associated β-galactosidase, telomere-length related genes, stemness, and mitochondrial metabolism. Using transcriptomic and proteomic analyses, we identified glucose-regulated protein 78 (GRP78) as a unique MSC senescence marker. With increasing cell passage number, GRP78 gradually translocated from the cell surface and cytosol to the (peri)nuclear region of TMSCs. A gelatin-based hydrogel releasing a sustained, low level of reactive oxygen species (ROS-hydrogel) was used to improve TMSC quiescence and self-renewal. TMSCs expressing cell surface-specific GRP78 (csGRP78+), collected by magnetic sorting, showed better stem cell function and higher mitochondrial metabolism than unsorted cells. Implantation of csGRP78+ cells embedded in ROS-hydrogel in rats with calvarial defects resulted in increased bone regeneration. Thus, csGRP78 is a promising biomarker of senescent TMSCs, and the combined use of csGRP78+ cells and ROS-hydrogel improved the regenerative capacity of TMSCs by regulating GRP78 translocation.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherElsevier Science-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHGlucose-
dc.subject.MESHHeat-Shock Proteins*-
dc.subject.MESHHydrogels-
dc.subject.MESHMembrane Proteins-
dc.subject.MESHMesenchymal Stem Cells*-
dc.subject.MESHOsteogenesis-
dc.subject.MESHPalatine Tonsil-
dc.subject.MESHProteomics-
dc.subject.MESHRats-
dc.subject.MESHReactive Oxygen Species*-
dc.titleTonsil-derived mesenchymal stem cells incorporated in reactive oxygen species-releasing hydrogel promote bone formation by increasing the translocation of cell surface GRP78-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorDa Hyeon Choi-
dc.contributor.googleauthorKyeong Eun Lee-
dc.contributor.googleauthorSe-Young Oh-
dc.contributor.googleauthorSi Min Lee-
dc.contributor.googleauthorBeom Soo Jo-
dc.contributor.googleauthorJue-Yeon Lee-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorYoon Jeong Park-
dc.contributor.googleauthorKi Dong Park-
dc.contributor.googleauthorInho Jo-
dc.contributor.googleauthorYoon Shin Park-
dc.identifier.doi10.1016/j.biomaterials.2021.121156-
dc.contributor.localIdA01662-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid34597900-
dc.subject.keywordBone regeneration-
dc.subject.keywordCell surface GRP78+-
dc.subject.keywordGlucose-regulated protein 78-
dc.subject.keywordROS releasing hydrogel-
dc.subject.keywordSenescence-
dc.subject.keywordTonsil-derived mesenchymal stem cells-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.affiliatedAuthor박종철-
dc.citation.volume278-
dc.citation.startPage121156-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.278 : 121156, 2021-11-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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