102 77

Cited 0 times in

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

Authors
 Minjeong Kim  ;  Sumin Hur  ;  Kwang H Kim  ;  Yejin Cho  ;  Keunyoung Kim  ;  Ha Ryong Kim  ;  Ki Taek Nam  ;  Kyung-Min Lim 
Citation
 BIOMOLECULES & THERAPEUTICS, Vol.30(2) : 126-136, 2022-03 
Journal Title
BIOMOLECULES & THERAPEUTICS
ISSN
 1976-9148 
Issue Date
2022-03
Keywords
IRAK3 ; Liver fibrosis ; Lumican ; Murine liver fibrosis model ; Polyhexamethylene guanidine phosphate (PHMG-p)
Abstract
Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of ~15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.
Files in This Item:
T202200569.pdf Download
DOI
10.4062/biomolther.2021.120
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Kwang Hui(김광휘)
Nam, Ki Taek(남기택)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188059
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links