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Cytokine Release from Organotypic Human Oral Tissue Following Exposure to Oral Care Product Chemicals

Other Titles
 Cytokine Release from Organotypic Human Oral Tissue Following Exposure to Oral Care Product Chemicals 
 Song-Yi Yang  ;  Yin-Zhu Piao  ;  KWANG MAHN KIM  ;  JaeSung Kwon 
 Journal of Korean Dental Science, Vol.14(2) : 51-60, 2021-12 
Journal Title
Journal of Korean Dental Science
Issue Date
Cytokines ; Dentistry ; Safety ; Tissues
Measuring viability of a three-dimensional in vitro organotypic human oral tissue model has been sug gested as an alternative test method to the oral mucosa irritation test of oral care products. The aim of this study was to investigate the production of two different cytokines using organotypic human oral tissue model following expo sure to chemicals that are commonly used in oral care products.
Materials and Methods: The organotypic human oral tissues were exposed to ethanol, sodium lauryl sulphate or hydrogen peroxide for 90 minutes. Following exposure, interleukin (IL)-1α and IL-8 productions were assessed and correlated with cell viability testing as well as histology of the organotypic human oral tissues.
Result: High levels of IL-8 were released from organotypic human oral tissues in all of the test and control groups without any significant differences between them. In contrast, differences were found in IL-1α release between the test and control groups. Additionally, the trend of IL-1α release corresponded to the phenotypes observed in histo logical analysis while different trend existed between IL-1α release and cell viability.
Conclusion: The study concluded the non-specific release of IL-8 for the assessment of oral care product chemicals’ toxicity, while potential of measuring IL-1α cytokine level as the possible alternative test method.
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
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