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The role of graphene patterning in field-effect transistor sensors to detect the tau protein for Alzheimer's disease: Simplifying the immobilization process and improving the performance of graphene-based immunosensors

Authors
 Sun Sang Kwon  ;  Dongwoo Kim  ;  Mijin Yun  ;  Jeong Gon Son  ;  Soo Hyun Lee 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.192 : 113519, 2021-11 
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
 0956-5663 
Issue Date
2021-11
MeSH
Alzheimer Disease* / diagnosis ; Biosensing Techniques* ; Graphite* ; Humans ; Immunoassay ; Transistors, Electronic ; tau Proteins
Keywords
Antibody immobilization ; Field-effect transistor sensor ; Graphene patterning ; Immunosensor ; Linker-free ; Tau protein
Abstract
We report the improvement in the sensing performance of electrolyte-gated graphene field-effect transistor (FET) sensors capable of detecting tau protein through a simplified, linker-free, anti-tau antibody immobilization process. For most of the graphene-based immunosensor, linkers, such as pyrenebutanoic acid, succinimidyl ester (PSE) must be used to the graphene surface, while the other side of linkers serves to capture the antibodies that can specifically interact with the target biomarker. In this study, graphene was patterned into eight different types and linker-free patterned graphene FET sensors were fabricated to verify their detection performance. The linker-free antibody immobilization to patterned graphene exhibited that the antibody was immobilized to the edge defect and had a doping-like behaviors on graphene. As the tau protein concentration in the electrolyte increased from 10 fg/ml to 1 ng/ml, the performances, charge neutral point shift and current change rate of the patterned graphene sensors without linkers were enhanced 2-3 times compared to a pristine graphene sensor with the PSE linker. Moreover, tau protein in the plasma of five Alzheimer's disease patients was measured using a linker-free patterned graphene sensor. It shows a 3-4 times higher current change rate than that of pristine graphene sensor with the PSE linker. Since the antibody is immobilized directly without a linker, a patterned graphene sensor without a linker can operate more sensitively in higher ionic concentration electrolyte.
Files in This Item:
T202125234.pdf Download
DOI
10.1016/j.bios.2021.113519
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dongwoo(김동우) ORCID logo https://orcid.org/0000-0002-1723-604X
Yun, Mi Jin(윤미진) ORCID logo https://orcid.org/0000-0002-1712-163X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187747
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