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TGF-β2 and collagen play pivotal roles in the spheroid formation and anti-aging of human dermal papilla cells

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dc.contributor.author송승용-
dc.date.accessioned2022-02-23T01:02:40Z-
dc.date.available2022-02-23T01:02:40Z-
dc.date.issued2021-08-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187509-
dc.description.abstractDermal papilla cells (DPCs) tend to aggregate both in vitro and in vivo, which increases the hair inductivity of DPCs. However, the underlying mechanism of spheroid formation is unknown. We investigated whether collagen expression in human DPCs (hDPCs) is involved in the spheroid formation and hair inductivity of hDPCs and further examined the underlying molecular mechanism of collagen upregulation. The expression of diverse collagens, such as COL13A1 and COL15A1, was upregulated in three dimensional (3D)-cultured or intact DPCs, compared to 2D-cultured hDPCs. This collagen expression was a downregulated in aged hair follicle, and aged DPCs were difficult to aggregate. Blocking of COL13A1 and COL15A1 by small interfering RNA reduced aggregation, while induced senescence of hDPCs in vitro. Further, transforming growth factor-β2 (TGF-β2) expression decreases with aging, and is involved in regulating the expression of COL13A1 and COL15A1. Addition of recombinant TGF-β2 delayed cellular senescence, and recovered spheroid formation in aged hDPCs by upregulating collagen levels. On the contrary, knock-out of TGF-β2 induced the aging of DPCs, and inhibited spheroid formation. These results suggested that COL13A1 and COL15A1 expression is downregulated with aging in DPCs, and upregulation of collagen by TGF-β2 induces the spheroid formation of DPCs. Therefore, TGF-β2 supplement in DPC culture medium could enhance the maintenance and hair inductivity of DPCs.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherImpact Journals-
dc.relation.isPartOfAging-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAging / genetics-
dc.subject.MESHAging / metabolism*-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCellular Senescence-
dc.subject.MESHCollagen / genetics-
dc.subject.MESHCollagen / metabolism*-
dc.subject.MESHCollagen Type XIII / genetics-
dc.subject.MESHCollagen Type XIII / metabolism*-
dc.subject.MESHDermis / cytology-
dc.subject.MESHDermis / metabolism*-
dc.subject.MESHHair Follicle / cytology-
dc.subject.MESHHair Follicle / metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHSpheroids, Cellular / cytology-
dc.subject.MESHSpheroids, Cellular / metabolism*-
dc.subject.MESHTransforming Growth Factor beta2 / genetics-
dc.subject.MESHTransforming Growth Factor beta2 / metabolism*-
dc.titleTGF-β2 and collagen play pivotal roles in the spheroid formation and anti-aging of human dermal papilla cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Plastic and Reconstructive Surgery (성형외과학교실)-
dc.contributor.googleauthorHyunju Kim-
dc.contributor.googleauthorNahyun Choi-
dc.contributor.googleauthorDoo Yeong Kim-
dc.contributor.googleauthorSo Yoon Kim-
dc.contributor.googleauthorSeung Yong Song-
dc.contributor.googleauthorJong-Hyuk Sung-
dc.identifier.doi10.18632/aging.203419-
dc.contributor.localIdA02032-
dc.relation.journalcodeJ03575-
dc.identifier.eissn1945-4589-
dc.identifier.pmid34404755-
dc.subject.keywordTGF-beta2-
dc.subject.keywordaggregation-
dc.subject.keywordanti-aging-
dc.subject.keywordcollagen-
dc.subject.keywordhuman dermal papilla cells-
dc.contributor.alternativeNameSong, Seung Yong-
dc.contributor.affiliatedAuthor송승용-
dc.citation.volume13-
dc.citation.number16-
dc.citation.startPage19978-
dc.citation.endPage19995-
dc.identifier.bibliographicCitationAging, Vol.13(16) : 19978-19995, 2021-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

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