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Runx3 regulates iron metabolism via modulation of BMP signalling

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dc.contributor.author이종민-
dc.contributor.author정한성-
dc.date.accessioned2022-02-23T00:55:09Z-
dc.date.available2022-02-23T00:55:09Z-
dc.date.issued2021-12-
dc.identifier.issn0960-7722-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187440-
dc.description.abstractObjectives: Runx3, a member of the Runx family of transcription factors, has been studied as a tumour suppressor and key player of organ development. In a previous study, we reported differentiation failure and excessive angiogenesis in the liver of Runx3 knock-out (KO) mice. Here, we examined a function of the Runx3 in liver, especially in iron metabolism. Methods: We performed histological and immunohistological analyses of the Runx3 KO mouse liver. RNA-sequencing analyses were performed on primary hepatocytes isolated from Runx3 conditional KO (cKO) mice. The effect of Runx3 knock-down (KD) was also investigated using siRNA-mediated KD in functional human hepatocytes and human hepatocellular carcinoma cells. Result: We observed an iron-overloaded liver with decreased expression of hepcidin in Runx3 KO mice. Expression of BMP6, a regulator of hepcidin transcription, and activity of the BMP pathway were decreased in the liver tissue of Runx3 KO mice. Transcriptome analysis on primary hepatocytes isolated from Runx3 cKO mice also revealed that iron-induced increase in BMP6 was mediated by Runx3. Similar results were observed in Runx3 knock-down experiments using HepaRG cells and HepG2 cells. Finally, we showed that Runx3 enhanced the activity of the BMP6 promoter by responding to iron stimuli in the hepatocytes. Conclusion: In conclusion, we suggest that Runx3 plays important roles in iron metabolism of the liver through regulation of BMP signalling.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherPublished for the Cell Kinetics Society-
dc.relation.isPartOfCELL PROLIFERATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHBone Morphogenetic Protein 6 / genetics-
dc.subject.MESHBone Morphogenetic Protein 6 / metabolism*-
dc.subject.MESHCore Binding Factor Alpha 3 Subunit / genetics-
dc.subject.MESHCore Binding Factor Alpha 3 Subunit / metabolism*-
dc.subject.MESHHep G2 Cells-
dc.subject.MESHHepatocytes / metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHLiver / metabolism*-
dc.subject.MESHMice-
dc.subject.MESHMice, Knockout-
dc.subject.MESHSignal Transduction*-
dc.titleRunx3 regulates iron metabolism via modulation of BMP signalling-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentOthers-
dc.contributor.googleauthorHyun-Yi Kim-
dc.contributor.googleauthorJong-Min Lee-
dc.contributor.googleauthorYou-Soub Lee-
dc.contributor.googleauthorShujin Li-
dc.contributor.googleauthorSeung-Jun Lee-
dc.contributor.googleauthorSuk-Chul Bae-
dc.contributor.googleauthorHan-Sung Jung-
dc.identifier.doi10.1111/cpr.13138-
dc.contributor.localIdA04640-
dc.contributor.localIdA03758-
dc.relation.journalcodeJ00487-
dc.identifier.eissn1365-2184-
dc.identifier.pmid34611951-
dc.subject.keywordBmp pathway-
dc.subject.keywordBmp6-
dc.subject.keywordRunx3-
dc.subject.keywordliver iron metabolism-
dc.contributor.alternativeNameLee, Jong Min-
dc.contributor.affiliatedAuthor이종민-
dc.contributor.affiliatedAuthor정한성-
dc.citation.volume54-
dc.citation.number12-
dc.citation.startPagee13138-
dc.identifier.bibliographicCitationCELL PROLIFERATION, Vol.54(12) : e13138, 2021-12-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers

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