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Activation of Wnt signalling reduces the population of cancer stem cells in ameloblastoma
DC Field | Value | Language |
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dc.contributor.author | 박진후 | - |
dc.contributor.author | 정영수 | - |
dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2021-12-28T17:12:43Z | - |
dc.date.available | 2021-12-28T17:12:43Z | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 0960-7722 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186989 | - |
dc.description.abstract | Objectives: The treatment of ameloblastoma, an odontogenic epithelial tumour destroying jawbone, mainly depends on radical destructive resections. Other therapeutic options are limited by the characteristics of ameloblastoma, such as high recurrence rates and resistance to radiation and chemotherapy, which implies possible existence of cancer stem cells (CSCs) in ameloblastoma. Here, we identified a putative CSC population in immortalized and primary human ameloblastoma cells and examined possible therapeutic reagents to reduce the CSC population. Methods: We investigated subpopulations of AM-1 cell line and human ameloblastoma cells using immunocytochemistry and flow cytometry and the effects of Wnt signalling activators on the 2- and 3-dimensional cultured ameloblastoma cells using molecular biological analyses. Result: Among heterogenous ameloblastoma cells, small-sized and round-shaped cells were found to be proliferative and expressed a marker of dental epithelial stem cells, SRY-box 2 (Sox2). Exogenous activation of Wnt signalling using glycogen synthase kinase 3β inhibitors, lithium chloride (LiCl) and valproic acid (VPA), increased the cell size and decreased proliferation of cells and expression of Sox2 in 2 dimensionally cultured AM-1 and human primary ameloblastoma cells. Furthermore, the growth of 3 dimensionally cultured AM-1 cells as suspended or embedded in gel was suppressed by treatment with Wnt signalling activators, VPA and CHIR99021, or antibodies to sclerostin, an antagonist of Wnt signalling. Conclusion: We suggest that Wnt signalling activators are potential drug candidates to suppress CSCs in ameloblastoma. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Published for the Cell Kinetics Society | - |
dc.relation.isPartOf | CELL PROLIFERATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Ameloblastoma / metabolism | - |
dc.subject.MESH | Ameloblastoma / pathology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation / drug effects | - |
dc.subject.MESH | Cell Size / drug effects | - |
dc.subject.MESH | Down-Regulation / drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lithium Chloride / pharmacology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Neoplastic Stem Cells / cytology | - |
dc.subject.MESH | Neoplastic Stem Cells / metabolism* | - |
dc.subject.MESH | SOXB1 Transcription Factors / metabolism | - |
dc.subject.MESH | Valproic Acid / pharmacology | - |
dc.subject.MESH | Wnt Signaling Pathway / drug effects* | - |
dc.subject.MESH | beta Catenin / metabolism | - |
dc.title | Activation of Wnt signalling reduces the population of cancer stem cells in ameloblastoma | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) | - |
dc.contributor.googleauthor | Hyun-Yi Kim | - |
dc.contributor.googleauthor | Shujin Li | - |
dc.contributor.googleauthor | Dong-Joon Lee | - |
dc.contributor.googleauthor | Jin Hoo Park | - |
dc.contributor.googleauthor | Takashi Muramatsu | - |
dc.contributor.googleauthor | Hidemitsu Harada | - |
dc.contributor.googleauthor | Young-Soo Jung | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.identifier.doi | 10.1111/cpr.13073 | - |
dc.contributor.localId | A05339 | - |
dc.contributor.localId | A03655 | - |
dc.contributor.localId | A03758 | - |
dc.relation.journalcode | J00487 | - |
dc.identifier.eissn | 1365-2184 | - |
dc.identifier.pmid | 34096124 | - |
dc.subject.keyword | Wnt signalling | - |
dc.subject.keyword | ameloblastoma | - |
dc.subject.keyword | cancer stem cell | - |
dc.contributor.alternativeName | Park, Jin Hoo | - |
dc.contributor.affiliatedAuthor | 박진후 | - |
dc.contributor.affiliatedAuthor | 정영수 | - |
dc.contributor.affiliatedAuthor | 정한성 | - |
dc.citation.volume | 54 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | e13073 | - |
dc.identifier.bibliographicCitation | CELL PROLIFERATION, Vol.54(7) : e13073, 2021-07 | - |
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