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Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells

Authors
 Jeong-Eun Yoo  ;  Dongjin R Lee  ;  Sanghyun Park  ;  Hye-Rim Shin  ;  Kun Gu Lee  ;  Dae-Sung Kim  ;  Mi-Young Jo  ;  Jang-Hyeon Eom  ;  Myung Soo Cho  ;  Dong-Youn Hwang  ;  Dong-Wook Kim 
Citation
 NPJ PARKINSONS DISEASE, Vol.7(1) : 61, 2021-07 
Journal Title
NPJ PARKINSONS DISEASE
ISSN
 2373-8057 
Issue Date
2021-07
Abstract
Successful cell therapy for Parkinson's disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Here, using LMX1A-eGFP knock-in reporter human embryonic stem cells, we discovered a novel surface antigen, trophoblast glycoprotein (TPBG), which was preferentially expressed in vmDA precursors. TPBG-targeted cell sorting enriched FOXA2+LMX1A+ vmDA precursors and helped attain efficient behavioral recovery of rodent PD models with increased numbers of TH+, NURR1+, and PITX3+ vmDA neurons in the grafts. Additionally, fewer proliferating cells were detected in TPBG+ cell-derived grafts than in TPBG- cell-derived grafts. Our approach is an efficient way to obtain enriched bona fide vmDA precursors, which could open a new avenue for effective PD treatment.
Files in This Item:
T202104882.pdf Download
DOI
10.1038/s41531-021-00204-8
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Park, Sang Hyun(박상현)
Yoo, Jeong Eun(유정은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186969
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