사람 비(鼻)점막 미세혈관내피세포의 배양 및 미세혈관 형성

Abstract

Human nasal microvascular endothelial cells were isolated and cultured from the inferior turbinate. Endothelial cells grew rapidly to confluence and survived serial passages until the eighth subculture in a medium composed of MCDB 107, 10% fetal bovine serum, 75μg/ml endothelial cell growth supplement and 24units/ml insulin in the gelatin-coated 60mm dish. The cells were identified as endothelial cells by their morphology and immunofluoresence stain for human factor Ⅷ antigen. Capillary-like tubule formation was usually noted 4-6weeks after confluence. We succeeded in long-term culture of human nasal microvascular endothelial cells from the inferior turbinate. This will provide good model for studying the role of endothelial cells.