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RAPD 표지자 분석에 의한 가시아메바속 한국분리주의 유전적 지위

Other Titles
 Genetic status of Acanthamoeba spp Korean isolates on the basis of RAPD markers 
Authors
 홍용표  ;  오승환  ;  라미숙  ;  임경일 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.33(4) : 341-348, 1995-12 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
1995-12
MeSH
Acanthamoeba / classification ; Acanthamoeba / genetics* ; Animals ; Korea ; Polymerase Chain Reaction ; Random Amplified Polymorphic DNA Technique*
Abstract
Genetic status of Acanthamoeba spp. were tested on the basis of random amplified polymorphic DNA (RAPD) marker analysis. Four previously established Acanthamoeba species, 4 Korean isolates of Acanthamoeba sp., and one American isolate of Acanthamoeba sp. were analyzed by RAPD- PCR using an arbitrary decamer primers. Amplification products were fractionated by agarose gel electrophoresis and stained by ethidium bromide. Eighteen primers produced DNA amplification profiles revealing clear differences among 4 species. Nine of them also produced DNA amplification profiles which included some isolate-specific amplification products. On the basis of amplified fragments by 18 primers, the pairwise similarity indices between A. culbertsoni and other species (i.e., A. hatchetti, A. triangularis, A. polyphaga) were 0.300, 0.308, and 0.313, respectively. Similarity index between A. hatchetti and A. triangularis was 0.833. The mean similarity index among the 3 Korean isolates (YM-2, -3, -4) was 0.959 and 0.832 among them and 2 other species (A. hatchetti and A. triangularis). The mean similarity index among YM-5 and other Korean isolates (YM-2, -3, -4) was 0.237. However, the similarity index between YM-5 and A. culbertsoni was 0.857, which suggests that YM-5 is genetically more similar to A. culbertsoni than other Korean isolates. Phenogram reconstructed by UPGMA method revealed that there are two groups: one group consists of A. hatchetti, A. triangularis, and 3 Korean isolates (YM-2, -3, -4), and the other group consists of A. culbertsoni, A. polyphaga, HOV, and YM-5.
Files in This Item:
T199501214.pdf Download
DOI
10.3347/kjp.1995.33.4.341
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186323
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