고온에서 IFN-gamma의 항바이러스 작용과 2'-5'oligosdenylate synthetase 활성도 변화

Abstract

In this report we performed plaque reduction test using Hep-2 cell and vesiculo-stomatitis virus(VSV) at 37℃and 39℃examine the effect of high temperature on the antiviral activity of human IFN-γ. And we measured the activity of 2-5A synthetase to determine the effect of high temperature on the 2-5A system of Hep-2 cells treated with IFN-α or IFN-γ at 39℃and at 37℃. In the case of the treatment of Hep-2 cells with IFN-α or IFN-γ before VSV inoculation, the antiviral actions of IFN-α and IFN-γwere greater at 39℃than at 37℃. In the case of INF-αor IFN-γ treatment after VSV inoculation, no antiviral action of IFN-α or IFN-γ was boserved. Enhancement of 2-5A synthetase activities by IFN-α or IFN-γ were greater at 39℃ than at 37℃. From this study, it may be concluded that the antiviral action of IFN-γand the activity of the 2-5A system are enhanced by high temperature. It seems that enhancement of the 2-5A system activity at high temperature may contribute to the enhancement of antiviral action of INF-γ. It is reported that different characters in 2-5A system activation by IFN-αor IFN-γ. Thus the mechanisms of enhancement of 2-5A system activation by IFN-γat high temperature should be further studyied.