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Localization of Intracellular Monoclonal Antibody Specific for Mycobacteria in Experimentally Induced Pulmonary Tuberculous Lesion
DC Field | Value | Language |
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dc.contributor.author | 김동수 | - |
dc.contributor.author | 김세종 | - |
dc.contributor.author | 신전수 | - |
dc.contributor.author | 이종두 | - |
dc.date.accessioned | 2021-12-27T16:51:49Z | - |
dc.date.available | 2021-12-27T16:51:49Z | - |
dc.date.issued | 1995-01 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186205 | - |
dc.description.abstract | To determine the intracellular localization of intravenously injected infection-specific monoclonal antibodies (mAbs) in the infected cells, immunohistochemical staining was carried out in an animal model having pulmonary tuberculous lesions induced by inoculation of heat-killed Mycobacterium tuberculosis H37Rv. One milligram of intact mouse mAb against mycobacteria (group I, n=10) and F(ab′)2 (group II, n=6) was intravenously injected to the rabbits of each group. Imrnunohistochemical staining using an antimouse Ab was performed at days 1, 3, 5, 7 and 8 in group I and at days 1, 2 and 3 in group II by the streptavidin-biotin method. For the control study, 1 ml of nonspecific polyclonal human IgG (group III, n=10) and 100 μg of normal rabbit IgG F(ab′)2 (group IV, n=6) was injected to rabbits and guinea pigs having tuberculous inflammation, respectively. Both groups (groups I and II) showed a positive antigen (Ag)-Ab reaction within the cytoplasm of monocytes. A weak but positive reaction was observed intracellularly in group III; however, no positive reaction was seen in group IV. Our results suggest that an intracellular Ag-Ab reaction plays an important role in the localization of infection by immunoscintigraphy using specific mAb fragments. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | S. Karger | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Bacterial / immunology* | - |
dc.subject.MESH | Antibodies, Monoclonal / immunology* | - |
dc.subject.MESH | Antigen-Antibody Reactions | - |
dc.subject.MESH | Guinea Pigs | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Mycobacterium tuberculosis / immunology* | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Radioimmunodetection | - |
dc.subject.MESH | Tuberculosis, Pulmonary / diagnosis* | - |
dc.subject.MESH | Tuberculosis, Pulmonary / pathology | - |
dc.title | Localization of Intracellular Monoclonal Antibody Specific for Mycobacteria in Experimentally Induced Pulmonary Tuberculous Lesion | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | ;Dept. of Pediatrics (소아과학교실) | - |
dc.contributor.googleauthor | Lee J.D. | - |
dc.contributor.googleauthor | Kim D.S. | - |
dc.contributor.googleauthor | Shin J.S. | - |
dc.contributor.googleauthor | Cho S.N. | - |
dc.contributor.googleauthor | Kim S.J. | - |
dc.contributor.googleauthor | Shin D.H. | - |
dc.contributor.googleauthor | Chung S.H. | - |
dc.contributor.googleauthor | Lee J.T. | - |
dc.contributor.googleauthor | Park C.Y. | - |
dc.contributor.googleauthor | Kim S.M. | - |
dc.identifier.doi | 10.1159/000236830 | - |
dc.contributor.localId | A00405 | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A02144 | - |
dc.contributor.localId | A03138 | - |
dc.relation.journalcode | J01074 | - |
dc.identifier.eissn | 1423-0097 | - |
dc.identifier.pmid | 7819738 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/236830 | - |
dc.contributor.alternativeName | Kim, Dong Soo | - |
dc.contributor.affiliatedAuthor | 김동수 | - |
dc.contributor.affiliatedAuthor | 김세종 | - |
dc.contributor.affiliatedAuthor | 신전수 | - |
dc.contributor.affiliatedAuthor | 이종두 | - |
dc.citation.volume | 106 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 113 | - |
dc.citation.endPage | 117 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.106(2) : 113-117, 1995-01 | - |
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