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이질아메바에 의한 인체 대장상피세포주 HT-29에서의 interleukin-8 유전자의 발현

Other Titles
 Interleukin-8 gene expression in the human colon epithelial cell line HT-29 exposed to Entamoeba histolytica 
Authors
 김정목  ;  정현채  ;  임경일  ;  조양자  ;  김정룡 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.33(4) : 357-364, 1995-12 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
1995-12
MeSH
Animals ; Cell Line ; Colon / metabolism* ; Entamoeba histolytica* ; Entamoebiasis / genetics* ; Entamoebiasis / metabolism ; Enzyme-Linked Immunosorbent Assay ; Epithelium / metabolism ; Gene Expression* ; Humans ; Interleukin-8 / genetics* ; Polymerase Chain Reaction ; RNA, Messenger / analysis
Abstract
The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoites for 30 minutes, 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1 × 107 molecules/µg of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results suggest that acute inflammatory reaction by E. histolytica may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.
Files in This Item:
T199500784.pdf Download
DOI
10.3347/kjp.1995.33.4.357
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186152
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