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In vivo Function of Differential Subsets of Cutaneous Dendritic Cells to Induce Th17 Immunity in Intradermal Candida albicans Infection

Authors
 Jeyun Park  ;  Sung Hee Kim  ;  Joohee Lee  ;  Lihua Che  ;  Won Seok Roh  ;  Chang Ook Park  ;  Min-Geol Lee  ;  Tae-Gyun Kim 
Citation
 JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Vol.173 : e62731, 2021-07 
Journal Title
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Issue Date
2021-07
Abstract
The skin is the outermost barrier organ in the body, which contains several types of dendritic cells (DCs), a group of professional antigen-presenting cells. When the skin encounters invading pathogens, different cutaneous DCs initiate a distinct T cell immune response to protect the body. Among the invading pathogens, fungal infection specifically drives a protective interleukin-17-producing Th17 immune response. A protocol was developed to efficiently differentiate Th17 cells by intradermal Candida albicans infection to investigate a subset of cutaneous DCs responsible for inducing Th17 immunity. Flow cytometry and gene expression analyses revealed a prominent induction of Th17 immune response in skin-draining lymph nodes and infected skin. Using diphtheria toxin-induced DC subset-depleting mouse strains, CD301b+ dermal DCs were found to be responsible for mounting optimal Th17 differentiation in this model. Thus, this protocol provides a valuable method to study in vivo function of differential subsets of cutaneous DCs to determine Th17 immunity against deep skin fungal infection.
Full Text
https://www.jove.com/t/62731/in-vivo-function-differential-subsets-cutaneous-dendritic-cells-to
DOI
10.3791/62731
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Tae-Gyun(김태균) ORCID logo https://orcid.org/0000-0002-2116-4579
Park, Chang Ook(박창욱) ORCID logo https://orcid.org/0000-0003-3856-1201
Lee, Min Geol(이민걸) ORCID logo https://orcid.org/0000-0001-7040-5335
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184787
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