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Korean red ginseng induces extrinsic and intrinsic apoptotic pathways in MCF-7 breast cancer cells and MCF-10A non-malignant breast cells

Authors
 Hye In Kim  ;  Inha Lee  ;  Yeon Soo Jung  ;  Seung Joo Chon  ;  Bo Hyon Yun  ;  Seok Kyo Seo 
Citation
 JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, Vol.47(8) : 2758-2766, 2021-08 
Journal Title
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
ISSN
 1341-8076 
Issue Date
2021-08
MeSH
Apoptosis ; Breast Neoplasms* / drug therapy ; Female ; Humans ; MCF-7 Cells ; Panax* ; Signal Transduction
Keywords
Korean red ginseng ; apoptosis ; breast ; menopause
Abstract
Background: Among non-hormonal treatments, herbal products are frequently used by women. Korean red ginseng (KRG) is one of the popular herbal medicines. KRG could be one option for relieving menopausal symptoms. However, there are still concerns about the safety for long-term use. In order to be used for alleviating menopausal symptoms, the safety of KRG on breast must be ensured. The purpose of this study was to investigate the effects of KRG on breast cells.

Methods: MCF-7 and MCF-10A cells were treated with different concentrations of KRG extracts for 48 h. Cell viability was evaluated by MTT assay, and apoptosis by flow cytometry. The expression of apoptosis-related proteins was determined by western blot analysis and estrogen receptor (ER) affinity by ER binding assay.

Results: KRG extract inhibited growth and induced apoptosis of both MCF-7 and MCF-10A cells in dose-dependent manner. KRG extract increased the expression of pro-apoptotic proteins BAX, BAK, and BAD and decreased the expression of anti-apoptotic proteins Bcl-2 and Bcl-XL in both cells. The expressions of Fas and FasL were increased in lower doses, but decreased in higher doses in both cells. Activities of caspase-3, -8 and -9 increased in MCF-10A, while caspase-8 and -9 showed increase in MCF-7. Competition of KRG to E2 was significant in MCF-7 as KRG dose increased, whereas ER binding was hardly shown in MCF-10.

Conclusion: KRG induced apoptosis via extrinsic and intrinsic pathway in MCF-7 breast cancer cells and MCF-10A non-malignant cells. KRG may be safely used with regard to breast cancer risk in postmenopausal women to reduce the vasomotor symptoms.
Full Text
https://obgyn.onlinelibrary.wiley.com/doi/10.1111/jog.14826
DOI
10.1111/jog.14826
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hye In(김혜인) ORCID logo https://orcid.org/0000-0001-7961-5988
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Yun, Bo Hyon(윤보현) ORCID logo https://orcid.org/0000-0001-5703-797X
Lee, Inha(이인하) ORCID logo https://orcid.org/0000-0003-4869-6281
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184697
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